TY - JOUR
T1 - Pulpal exposure alters neuropeptide levels in inflamed dental pulp and trigeminal ganglia
T2 - Evaluation of axonal transport
AU - Buck-Wischmeier, Susan
AU - Reese, Karen
AU - Hargreaves, Kenneth M.
N1 - Funding Information:
This study was funded in part by DE10096 and P30DE09737, and by a UROP grant (to S.B.).
PY - 1999/11
Y1 - 1999/11
N2 - Dental pulp is richly innervated with neurons containing calcitonin gene-related peptide (CGRP) or substance P (SP). Prior studies have demonstrated that inflammation alters these pulpal neuropeptides. In this study, we used a radioimmunoassay to evaluate the specificity of this response and the contribution of axonal transport. Rat mandibular molars were exposed and immunoreactive CGRP (iCGRP) and immunoreactive SP were measured. At 7 to 14 days after exposure, both pulpal iCGRP (73%) and immunoreactive SP (135%) displayed peak increases above control levels. This response was somatotopically restricted, with no changes observed in contralateral (intact) molars, or in ipsilateral mandibular molars after exposure of maxillary molars. Transection of the inferior alveolar nerve on day 13 significantly reduced pulpal levels of iCGRP on day 14. Collectively, these studies indicate that pulpal inflammation evokes a selective alteration in neuropeptide levels, due at least in part to alterations in transport or synthesis of neuropeptides in the trigeminal ganglion.
AB - Dental pulp is richly innervated with neurons containing calcitonin gene-related peptide (CGRP) or substance P (SP). Prior studies have demonstrated that inflammation alters these pulpal neuropeptides. In this study, we used a radioimmunoassay to evaluate the specificity of this response and the contribution of axonal transport. Rat mandibular molars were exposed and immunoreactive CGRP (iCGRP) and immunoreactive SP were measured. At 7 to 14 days after exposure, both pulpal iCGRP (73%) and immunoreactive SP (135%) displayed peak increases above control levels. This response was somatotopically restricted, with no changes observed in contralateral (intact) molars, or in ipsilateral mandibular molars after exposure of maxillary molars. Transection of the inferior alveolar nerve on day 13 significantly reduced pulpal levels of iCGRP on day 14. Collectively, these studies indicate that pulpal inflammation evokes a selective alteration in neuropeptide levels, due at least in part to alterations in transport or synthesis of neuropeptides in the trigeminal ganglion.
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U2 - 10.1016/S0099-2399(99)80116-2
DO - 10.1016/S0099-2399(99)80116-2
M3 - Article
C2 - 10726536
AN - SCOPUS:0033223804
SN - 0099-2399
VL - 25
SP - 718
EP - 721
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 11
ER -