Purification of the major histocompatibility complex class I transcription factor H2TF1. The full-length product of the nfkb2 gene

D. A. Potter, C. J. Larson, P. Eckes, R. M. Schmid, G. J. Nabel, G. L. Verdine, P. A. Sharp

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18 Scopus citations

Abstract

H2TF1 is a ubiquitous major histocompatibility complex (MHC) class I- specific transcription factor, which binds to the palindromic κB enhancer site upstream of MHC class I genes. Here we report that H2TF1 consists of a polypeptide with relative molecular mass 110,000, that corresponds to the predicted 100-kDa product (NF-κB2 p100) encoded by the candidate proto- oncogene nfkb2 (lyt-10). H2TF1 was purified by a novel affinity chromatography method and identified as the NF-κB2 p100 polypeptide by peptide sequencing as well as by reactivity with a specific antiserum. Purified H2TF1 binds the MHC κB site with high affinity (K(D) = 3 x 10- 11 M), in contrast with previous reports that NF-κB2 p100 did not bind DNA.

Original languageEnglish (US)
Pages (from-to)18882-18890
Number of pages9
JournalJournal of Biological Chemistry
Volume268
Issue number25
StatePublished - 1993

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