H2TF1 is a ubiquitous major histocompatibility complex (MHC) class I- specific transcription factor, which binds to the palindromic κB enhancer site upstream of MHC class I genes. Here we report that H2TF1 consists of a polypeptide with relative molecular mass 110,000, that corresponds to the predicted 100-kDa product (NF-κB2 p100) encoded by the candidate proto- oncogene nfkb2 (lyt-10). H2TF1 was purified by a novel affinity chromatography method and identified as the NF-κB2 p100 polypeptide by peptide sequencing as well as by reactivity with a specific antiserum. Purified H2TF1 binds the MHC κB site with high affinity (K(D) = 3 x 10- 11 M), in contrast with previous reports that NF-κB2 p100 did not bind DNA.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of Biological Chemistry|
|State||Published - 1993|