QTL mapping of adult plant resistance to Ug99 stem rust in the spring wheat population RB07/MN06113-8

Prabin Bajgain; Matthew N Rouse; Sridhar Bhavani; James A Anderson

doi:10.1007/s11032-015-0362-x

QTL mapping of adult plant resistance to Ug99 stem rust in the spring wheat population RB07/MN06113-8

Prabin Bajgain, Matthew N Rouse, Sridhar Bhavani, James A Anderson

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The emergence and spread of the Ug99 race group of the stem rust pathogen (Puccinia graminis Pers. f. sp. tritici) in the past decade have exposed the vulnerability of wheat (Triticum aestivum L.) to this disease. Discovery of novel and effective sources of resistance is vital for breeding resistant varieties to avert losses. The experimental breeding line MN06113-8 and cultivar RB07 developed by the University of Minnesota wheat breeding program exhibited adult plant resistance (APR) to the Ug99 race group in field tests in Kenya and Ethiopia. Both lines were found to be susceptible at the seedling stage to isolates of the race TTKSK, TTKST, and TTTSK. To dissect the genetic mechanism of resistance present in these lines, MN06113-8 was crossed to RB07 to generate 141 F₆ recombinant inbred lines (RILs). The RIL population was evaluated for APR to Ug99 in Kenya and Ethiopia over three seasons and for resistance to North American stem rust pathogen races in St. Paul, MN, in one season. The population was genotyped using high-throughput SNP genotyping assays. Composite interval mapping detected six quantitative trait loci (QTL) involved in APR to African stem rust races and three QTLs involved in stem rust resistance to North American stem rust races. One QTL located on chromosome 2B was associated with APR to stem rust races in all environments. Development of diagnostic markers linked to this gene will facilitate marker-assisted selection of resistant lines to develop varieties with enhanced levels of stem rust resistance.

Original language	English (US)
Article number	170
Journal	Molecular Breeding
Volume	35
Issue number	8
DOIs	https://doi.org/10.1007/s11032-015-0362-x
State	Published - Aug 6 2015

Bibliographical note

Funding Information:
We thank the University of Minnesota Genomics Center, University of Minnesota Supercomputing Institute, the Microbial & Plant Genomics Institute, the University of Minnesota Graduate School, Kenya Agriculture and Livestock Research Organization, Ethiopia Institute of Agricultural Research, USDA-ARS Cereal Disease Laboratory personnel, and Anderson Wheat Lab for their help and support during various phases of the project. Funding for this work was provided by the United States Department of Agriculture, Agriculture and Food Research Initiative and 2011-68002-30029 (Triticeae Coordinated Agricultural Project, www.triticeaecap.org ), the Borlaug Global Rust Initiative Durable Rust Resistance in Wheat Project (administered by Cornell University with a grant from the Bill & Melinda Gates Foundation), and the UK Department for International Development.

Publisher Copyright:
© 2015, Springer Science+Business Media Dordrecht.

Keywords

Genotyping by sequencing
Linkage mapping
Recombinant inbred lines
Resistance breeding
Stem rust
Ug99

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1007/s11032-015-0362-x

OpenUrl availability

Full text

Cite this

@article{a44be561ee464675bd24f53b01397739,

title = "QTL mapping of adult plant resistance to Ug99 stem rust in the spring wheat population RB07/MN06113-8",

abstract = "The emergence and spread of the Ug99 race group of the stem rust pathogen (Puccinia graminis Pers. f. sp. tritici) in the past decade have exposed the vulnerability of wheat (Triticum aestivum L.) to this disease. Discovery of novel and effective sources of resistance is vital for breeding resistant varieties to avert losses. The experimental breeding line MN06113-8 and cultivar RB07 developed by the University of Minnesota wheat breeding program exhibited adult plant resistance (APR) to the Ug99 race group in field tests in Kenya and Ethiopia. Both lines were found to be susceptible at the seedling stage to isolates of the race TTKSK, TTKST, and TTTSK. To dissect the genetic mechanism of resistance present in these lines, MN06113-8 was crossed to RB07 to generate 141 F6 recombinant inbred lines (RILs). The RIL population was evaluated for APR to Ug99 in Kenya and Ethiopia over three seasons and for resistance to North American stem rust pathogen races in St. Paul, MN, in one season. The population was genotyped using high-throughput SNP genotyping assays. Composite interval mapping detected six quantitative trait loci (QTL) involved in APR to African stem rust races and three QTLs involved in stem rust resistance to North American stem rust races. One QTL located on chromosome 2B was associated with APR to stem rust races in all environments. Development of diagnostic markers linked to this gene will facilitate marker-assisted selection of resistant lines to develop varieties with enhanced levels of stem rust resistance.",

keywords = "Genotyping by sequencing, Linkage mapping, Recombinant inbred lines, Resistance breeding, Stem rust, Ug99",

author = "Prabin Bajgain and Rouse, {Matthew N} and Sridhar Bhavani and Anderson, {James A}",

note = "Funding Information: We thank the University of Minnesota Genomics Center, University of Minnesota Supercomputing Institute, the Microbial & Plant Genomics Institute, the University of Minnesota Graduate School, Kenya Agriculture and Livestock Research Organization, Ethiopia Institute of Agricultural Research, USDA-ARS Cereal Disease Laboratory personnel, and Anderson Wheat Lab for their help and support during various phases of the project. Funding for this work was provided by the United States Department of Agriculture, Agriculture and Food Research Initiative and 2011-68002-30029 (Triticeae Coordinated Agricultural Project, www.triticeaecap.org ), the Borlaug Global Rust Initiative Durable Rust Resistance in Wheat Project (administered by Cornell University with a grant from the Bill & Melinda Gates Foundation), and the UK Department for International Development. Publisher Copyright: {\textcopyright} 2015, Springer Science+Business Media Dordrecht.",

year = "2015",

month = aug,

day = "6",

doi = "10.1007/s11032-015-0362-x",

language = "English (US)",

volume = "35",

journal = "Molecular Breeding",

issn = "1380-3743",

publisher = "Springer Netherlands",

number = "8",

}

TY - JOUR

T1 - QTL mapping of adult plant resistance to Ug99 stem rust in the spring wheat population RB07/MN06113-8

AU - Bajgain, Prabin

AU - Rouse, Matthew N

AU - Bhavani, Sridhar

AU - Anderson, James A

N1 - Funding Information: We thank the University of Minnesota Genomics Center, University of Minnesota Supercomputing Institute, the Microbial & Plant Genomics Institute, the University of Minnesota Graduate School, Kenya Agriculture and Livestock Research Organization, Ethiopia Institute of Agricultural Research, USDA-ARS Cereal Disease Laboratory personnel, and Anderson Wheat Lab for their help and support during various phases of the project. Funding for this work was provided by the United States Department of Agriculture, Agriculture and Food Research Initiative and 2011-68002-30029 (Triticeae Coordinated Agricultural Project, www.triticeaecap.org ), the Borlaug Global Rust Initiative Durable Rust Resistance in Wheat Project (administered by Cornell University with a grant from the Bill & Melinda Gates Foundation), and the UK Department for International Development. Publisher Copyright: © 2015, Springer Science+Business Media Dordrecht.

PY - 2015/8/6

Y1 - 2015/8/6

N2 - The emergence and spread of the Ug99 race group of the stem rust pathogen (Puccinia graminis Pers. f. sp. tritici) in the past decade have exposed the vulnerability of wheat (Triticum aestivum L.) to this disease. Discovery of novel and effective sources of resistance is vital for breeding resistant varieties to avert losses. The experimental breeding line MN06113-8 and cultivar RB07 developed by the University of Minnesota wheat breeding program exhibited adult plant resistance (APR) to the Ug99 race group in field tests in Kenya and Ethiopia. Both lines were found to be susceptible at the seedling stage to isolates of the race TTKSK, TTKST, and TTTSK. To dissect the genetic mechanism of resistance present in these lines, MN06113-8 was crossed to RB07 to generate 141 F6 recombinant inbred lines (RILs). The RIL population was evaluated for APR to Ug99 in Kenya and Ethiopia over three seasons and for resistance to North American stem rust pathogen races in St. Paul, MN, in one season. The population was genotyped using high-throughput SNP genotyping assays. Composite interval mapping detected six quantitative trait loci (QTL) involved in APR to African stem rust races and three QTLs involved in stem rust resistance to North American stem rust races. One QTL located on chromosome 2B was associated with APR to stem rust races in all environments. Development of diagnostic markers linked to this gene will facilitate marker-assisted selection of resistant lines to develop varieties with enhanced levels of stem rust resistance.

AB - The emergence and spread of the Ug99 race group of the stem rust pathogen (Puccinia graminis Pers. f. sp. tritici) in the past decade have exposed the vulnerability of wheat (Triticum aestivum L.) to this disease. Discovery of novel and effective sources of resistance is vital for breeding resistant varieties to avert losses. The experimental breeding line MN06113-8 and cultivar RB07 developed by the University of Minnesota wheat breeding program exhibited adult plant resistance (APR) to the Ug99 race group in field tests in Kenya and Ethiopia. Both lines were found to be susceptible at the seedling stage to isolates of the race TTKSK, TTKST, and TTTSK. To dissect the genetic mechanism of resistance present in these lines, MN06113-8 was crossed to RB07 to generate 141 F6 recombinant inbred lines (RILs). The RIL population was evaluated for APR to Ug99 in Kenya and Ethiopia over three seasons and for resistance to North American stem rust pathogen races in St. Paul, MN, in one season. The population was genotyped using high-throughput SNP genotyping assays. Composite interval mapping detected six quantitative trait loci (QTL) involved in APR to African stem rust races and three QTLs involved in stem rust resistance to North American stem rust races. One QTL located on chromosome 2B was associated with APR to stem rust races in all environments. Development of diagnostic markers linked to this gene will facilitate marker-assisted selection of resistant lines to develop varieties with enhanced levels of stem rust resistance.

KW - Genotyping by sequencing

KW - Linkage mapping

KW - Recombinant inbred lines

KW - Resistance breeding

KW - Stem rust

KW - Ug99

UR - http://www.scopus.com/inward/record.url?scp=84938559682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938559682&partnerID=8YFLogxK

U2 - 10.1007/s11032-015-0362-x

DO - 10.1007/s11032-015-0362-x

M3 - Article

AN - SCOPUS:84938559682

SN - 1380-3743

VL - 35

JO - Molecular Breeding

JF - Molecular Breeding

IS - 8

M1 - 170

ER -

QTL mapping of adult plant resistance to Ug99 stem rust in the spring wheat population RB07/MN06113-8

Abstract

Bibliographical note

Keywords

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this