Quantification of singlet oxygen production in the reaction of superoxide with hydrogen peroxide using a selective chemiluminescent probe

Laura A. MacManus-Spencer, Kristopher McNeill

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

A sensitive chemiluminescent probe that selectively reacts with singlet oxygen in the presence of superoxide and hydrogen peroxide has been used to quantify the production of singlet oxygen in the reaction of superoxide with hydrogen peroxide. The yield of singlet oxygen from this reaction was found to be low (0.2% relative to the initial superoxide concentration). No evidence for the formation of hydroxyl radical was observed in this reaction, ruling out the Haber-Weiss mechanism as a major singlet oxygen formation pathway. No singlet oxygen production was observed in the reaction of superoxide with 2-nitrobenzoic acid, which has a pKa similar to that of hydrogen peroxide, rendering the protonation of superoxide, followed by its disproportionation, an unlikely explanation for the formation of singlet oxygen in this system. The low yields of singlet oxygen and hydroxyl radical suggest that their formation in this reaction should be relatively unimportant in biological systems.

Original languageEnglish (US)
Pages (from-to)8954-8955
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number25
DOIs
StatePublished - Jun 29 2005

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