Quantification of tryptamine in brain using high-performance liquid chromatography

Alice A. Larson; Nelson L. Dalo

doi:10.1016/S0378-4347(00)83689-0

Quantification of tryptamine in brain using high-performance liquid chromatography

Alice A. Larson, Nelson L. Dalo

Veterinary and Biomedical Sciences

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Abstract

The concentration of endogenously formed tryptamine in central nervous system tissue was determined after extraction into ethyl acetate, purification on a weak cation-exchange resin and analysis using high-performance liquid chromatography with fluorometric detection. Final chromatographic separation of this indoleamine was achieved using a μBondapak C₁₈ reversed-phase column under isocratic conditions. Using this method, the concentration of tryptamine in the whole brain of normal rats was found to be 0.60 ± 0.06 ng/g of tissue, while pretreatment with dl-p-chlorophenylalanine, trnylcypromine and l-tryptophan increased the concentration to 96.7 ± 21.9 ng/g.

Original language	English (US)
Pages (from-to)	37-47
Number of pages	11
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	375
Issue number	C
DOIs	https://doi.org/10.1016/S0378-4347(00)83689-0
State	Published - 1986

Bibliographical note

Funding Information:
The authors wish to thank MS Suzanne Welthers for her excellent technical assistance and suggestionsd urmg the development of this assay This research was supported by NIH Grant NS17407

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title = "Quantification of tryptamine in brain using high-performance liquid chromatography",

abstract = "The concentration of endogenously formed tryptamine in central nervous system tissue was determined after extraction into ethyl acetate, purification on a weak cation-exchange resin and analysis using high-performance liquid chromatography with fluorometric detection. Final chromatographic separation of this indoleamine was achieved using a μBondapak C18 reversed-phase column under isocratic conditions. Using this method, the concentration of tryptamine in the whole brain of normal rats was found to be 0.60 ± 0.06 ng/g of tissue, while pretreatment with dl-p-chlorophenylalanine, trnylcypromine and l-tryptophan increased the concentration to 96.7 ± 21.9 ng/g.",

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T1 - Quantification of tryptamine in brain using high-performance liquid chromatography

AU - Larson, Alice A.

AU - Dalo, Nelson L.

N1 - Funding Information: The authors wish to thank MS Suzanne Welthers for her excellent technical assistance and suggestionsd urmg the development of this assay This research was supported by NIH Grant NS17407

PY - 1986

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N2 - The concentration of endogenously formed tryptamine in central nervous system tissue was determined after extraction into ethyl acetate, purification on a weak cation-exchange resin and analysis using high-performance liquid chromatography with fluorometric detection. Final chromatographic separation of this indoleamine was achieved using a μBondapak C18 reversed-phase column under isocratic conditions. Using this method, the concentration of tryptamine in the whole brain of normal rats was found to be 0.60 ± 0.06 ng/g of tissue, while pretreatment with dl-p-chlorophenylalanine, trnylcypromine and l-tryptophan increased the concentration to 96.7 ± 21.9 ng/g.

AB - The concentration of endogenously formed tryptamine in central nervous system tissue was determined after extraction into ethyl acetate, purification on a weak cation-exchange resin and analysis using high-performance liquid chromatography with fluorometric detection. Final chromatographic separation of this indoleamine was achieved using a μBondapak C18 reversed-phase column under isocratic conditions. Using this method, the concentration of tryptamine in the whole brain of normal rats was found to be 0.60 ± 0.06 ng/g of tissue, while pretreatment with dl-p-chlorophenylalanine, trnylcypromine and l-tryptophan increased the concentration to 96.7 ± 21.9 ng/g.

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Quantification of tryptamine in brain using high-performance liquid chromatography

Abstract

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