The concentration of endogenously formed tryptamine in central nervous system tissue was determined after extraction into ethyl acetate, purification on a weak cation-exchange resin and analysis using high-performance liquid chromatography with fluorometric detection. Final chromatographic separation of this indoleamine was achieved using a μBondapak C18 reversed-phase column under isocratic conditions. Using this method, the concentration of tryptamine in the whole brain of normal rats was found to be 0.60 ± 0.06 ng/g of tissue, while pretreatment with dl-p-chlorophenylalanine, trnylcypromine and l-tryptophan increased the concentration to 96.7 ± 21.9 ng/g.
|Original language||English (US)|
|Number of pages||11|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|State||Published - 1986|
Bibliographical noteFunding Information:
The authors wish to thank MS Suzanne Welthers for her excellent technical assistance and suggestionsd urmg the development of this assay This research was supported by NIH Grant NS17407