Quantitative assessment of cochlear and vestibular ganglion neurons in temporal bones with chronic otitis media

Rafael da Costa Monsanto; Norma de Oliveira Penido; Mio Uchiyama; Patricia Schachern; Michael Paparella; Sebahattin Cureoglu

doi:10.1007/s00405-020-06094-5

Quantitative assessment of cochlear and vestibular ganglion neurons in temporal bones with chronic otitis media

Rafael da Costa Monsanto, Norma de Oliveira Penido, Mio Uchiyama, Patricia Schachern, Michael Paparella, Sebahattin Cureoglu

Otolaryngology

Research output: Contribution to journal › Article › peer-review

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Abstract

Purpose: In this study, we aimed to determine whether or not COM leads to loss of spiral and Scarpa ganglion neurons. Methods: From the human temporal bone (HTB) collection at the University of Minnesota we selected human temporal bones with COM, defined as the presence of clinically intractable tissue abnormalities in the middle ear (cholesteatoma, perforation of the eardrum, granulation tissue, fibrosis, tympanosclerosis, and cholesterol granuloma). We also selected HTBs from donors with no ear diseases as controls. We quantitatively analyzed the number of spiral and Scarpa ganglion cells and compared the results obtained in the control and study groups. Results: In both COM and control groups we observed a significant negative correlation between age and number of both spiral (R = -0.632; P < 0.001; 95% CI − 0.766 to − 0.434) and Scarpa ganglion (R = − 0.404; P = 0.008; 95% CI − 0.636 to − 0.051) cells. We did not find any significant differences in the number of spiral ganglion cells (in total or per segment) or in the density of Scarpa ganglion cells (in each vestibular nerve or both) in the COM group as compared with controls (P > 0.05). Conclusions and relevance: Our results did not demonstrate significant loss of cochlear or vestibular peripheral ganglion neuron loss in HTBs with COM as compared with controls.

Original language	English (US)
Pages (from-to)	331-338
Number of pages	8
Journal	European Archives of Oto-Rhino-Laryngology
Volume	278
Issue number	2
DOIs	https://doi.org/10.1007/s00405-020-06094-5
State	Published - Feb 2021

Bibliographical note

Funding Information:
We thank our supporters: the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES—Brazil) (Finance code: 001), the National Institute of Neurological Disorders and Stroke of the NIH (Grant No. UG3NS107688), the International Hearing Foundation, the 5 M Lions International, and the Starkey Foundation.

Funding Information:
RCM received a scholarship from the “Coordenação de aperfeiçoamento pessoal de nível superior” (CAPES) (Finance code: 001). The research reported in this manuscript was supported by the National Institute of Neurological Disorders and Stroke of the NIH (UG3NS107688), the International Hearing Foundation, the 5 M Lions International, and the Starkey Foundation. Acknowledgements

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Chronic otitis media
Hearing loss
Otitis media
Scarpa ganglion cells
Spiral ganglion cells
Tinnitus
Vestibular diseases

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title = "Quantitative assessment of cochlear and vestibular ganglion neurons in temporal bones with chronic otitis media",

abstract = "Purpose: In this study, we aimed to determine whether or not COM leads to loss of spiral and Scarpa ganglion neurons. Methods: From the human temporal bone (HTB) collection at the University of Minnesota we selected human temporal bones with COM, defined as the presence of clinically intractable tissue abnormalities in the middle ear (cholesteatoma, perforation of the eardrum, granulation tissue, fibrosis, tympanosclerosis, and cholesterol granuloma). We also selected HTBs from donors with no ear diseases as controls. We quantitatively analyzed the number of spiral and Scarpa ganglion cells and compared the results obtained in the control and study groups. Results: In both COM and control groups we observed a significant negative correlation between age and number of both spiral (R = -0.632; P < 0.001; 95% CI − 0.766 to − 0.434) and Scarpa ganglion (R = − 0.404; P = 0.008; 95% CI − 0.636 to − 0.051) cells. We did not find any significant differences in the number of spiral ganglion cells (in total or per segment) or in the density of Scarpa ganglion cells (in each vestibular nerve or both) in the COM group as compared with controls (P > 0.05). Conclusions and relevance: Our results did not demonstrate significant loss of cochlear or vestibular peripheral ganglion neuron loss in HTBs with COM as compared with controls.",

keywords = "Chronic otitis media, Hearing loss, Otitis media, Scarpa ganglion cells, Spiral ganglion cells, Tinnitus, Vestibular diseases",

author = "Monsanto, {Rafael da Costa} and Penido, {Norma de Oliveira} and Mio Uchiyama and Patricia Schachern and Michael Paparella and Sebahattin Cureoglu",

note = "Funding Information: We thank our supporters: the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior (CAPES—Brazil) (Finance code: 001), the National Institute of Neurological Disorders and Stroke of the NIH (Grant No. UG3NS107688), the International Hearing Foundation, the 5 M Lions International, and the Starkey Foundation. Funding Information: RCM received a scholarship from the “Coordena{\c c}{\~a}o de aperfei{\c c}oamento pessoal de n{\'i}vel superior” (CAPES) (Finance code: 001). The research reported in this manuscript was supported by the National Institute of Neurological Disorders and Stroke of the NIH (UG3NS107688), the International Hearing Foundation, the 5 M Lions International, and the Starkey Foundation. Acknowledgements Publisher Copyright: {\textcopyright} 2020, Springer-Verlag GmbH Germany, part of Springer Nature.",

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AU - Monsanto, Rafael da Costa

AU - Penido, Norma de Oliveira

AU - Uchiyama, Mio

AU - Schachern, Patricia

AU - Paparella, Michael

AU - Cureoglu, Sebahattin

N1 - Funding Information: We thank our supporters: the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES—Brazil) (Finance code: 001), the National Institute of Neurological Disorders and Stroke of the NIH (Grant No. UG3NS107688), the International Hearing Foundation, the 5 M Lions International, and the Starkey Foundation. Funding Information: RCM received a scholarship from the “Coordenação de aperfeiçoamento pessoal de nível superior” (CAPES) (Finance code: 001). The research reported in this manuscript was supported by the National Institute of Neurological Disorders and Stroke of the NIH (UG3NS107688), the International Hearing Foundation, the 5 M Lions International, and the Starkey Foundation. Acknowledgements Publisher Copyright: © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2021/2

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N2 - Purpose: In this study, we aimed to determine whether or not COM leads to loss of spiral and Scarpa ganglion neurons. Methods: From the human temporal bone (HTB) collection at the University of Minnesota we selected human temporal bones with COM, defined as the presence of clinically intractable tissue abnormalities in the middle ear (cholesteatoma, perforation of the eardrum, granulation tissue, fibrosis, tympanosclerosis, and cholesterol granuloma). We also selected HTBs from donors with no ear diseases as controls. We quantitatively analyzed the number of spiral and Scarpa ganglion cells and compared the results obtained in the control and study groups. Results: In both COM and control groups we observed a significant negative correlation between age and number of both spiral (R = -0.632; P < 0.001; 95% CI − 0.766 to − 0.434) and Scarpa ganglion (R = − 0.404; P = 0.008; 95% CI − 0.636 to − 0.051) cells. We did not find any significant differences in the number of spiral ganglion cells (in total or per segment) or in the density of Scarpa ganglion cells (in each vestibular nerve or both) in the COM group as compared with controls (P > 0.05). Conclusions and relevance: Our results did not demonstrate significant loss of cochlear or vestibular peripheral ganglion neuron loss in HTBs with COM as compared with controls.

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Quantitative assessment of cochlear and vestibular ganglion neurons in temporal bones with chronic otitis media

Abstract

Bibliographical note

Keywords

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