Abstract
Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is provided in which the fluorescence resonance energy transfer between a donor-acceptor pair of fluorescent proteins within a cellular specimen is measured.
Original language | English (US) |
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Pages (from-to) | 362-367 |
Number of pages | 6 |
Journal | Journal of biomedical optics |
Volume | 8 |
Issue number | 3 |
DOIs | |
State | Published - Jul 2003 |
Keywords
- Fluorescence lifetime imaging microscopy