Quercetin-3-O-β-d-glucopyranosyl-(1→6)-β-d-glucopyranoside suppresses melanin synthesis by augmenting p38 MAPK and CREB signaling pathways and subsequent cAMP down-regulation in murine melanoma cells

Hyun Gug Jung, Han Hyuk Kim, Souren Paul, Jae Yoon Jang, Yong Hun Cho, Hyeon Jeong Kim, Jae Myo Yu, Eun Su Lee, Bong Jeun An, Sun Chul Kang, Byung Ho Bang

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, the effect of purified quercetin-3-O-β- d-glucopyranosyl-(1. →. 6)-β- d-glucopyranosid (QCGG) on melanogenesis was investigated. QCGG was isolated from the calyx of a traditional Korean medicinal herb, Persimmon (Diospyros kaki). The hypopigmentation effects of QCGG were determined by examination of cellular melanin contents, tyrosinase activity assay, cAMP assay, and Western blotting of α-MSH-stimulated B16F10 mouse melanoma cells. Our results showed that QCGG inhibited both melanin synthesis and tyrosinase activity in a concentration-dependent manner as well as significantly reduced the expression of melanogenic proteins such as microphthalmia-associated transcription factor (MITF), tyrosinase-related protein-1, tyrosinase-related protein-2, and tyrosinase. Moreover, QCGG inhibited intracellular cAMP levels, cAMP response element-binding protein (CREB), and p38 MAPK expression in α-MSH-stimulated B16F10 cells. Taken together, the suppressive effects of QCGG on melanogenesis may involve down-regulation of MITF and its downstream signaling pathway via phosphorylation of p38 MAPK and CREB along with reduced cAMP levels. These results indicate that QCGG reduced melanin synthesis by reducing expression of tyrosine and tyrosine-related proteins via extracellular signal-related protein kinase (ERK) activation, followed by down-regulation of CREB, p38, and MITF.

Original languageEnglish (US)
Pages (from-to)706-713
Number of pages8
JournalSaudi Journal of Biological Sciences
Volume22
Issue number6
DOIs
StatePublished - Nov 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 The Authors.

Keywords

  • CAMP
  • CREB
  • Melanogenesis
  • P38 MAPK
  • Persimmon calyx
  • TRPs

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