TY - JOUR
T1 - Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR
AU - Johnson, James R.
AU - Owens, Krista
AU - Sabate, Montse
AU - Prats, Guillem
PY - 2004/8
Y1 - 2004/8
N2 - Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.
AB - Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.
UR - http://www.scopus.com/inward/record.url?scp=3843111245&partnerID=8YFLogxK
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U2 - 10.1128/JCM.42.8.3841-3843.2004
DO - 10.1128/JCM.42.8.3841-3843.2004
M3 - Article
C2 - 15297544
AN - SCOPUS:3843111245
SN - 0095-1137
VL - 42
SP - 3841
EP - 3843
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 8
ER -