Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR

James R. Johnson; Krista Owens; Montse Sabate; Guillem Prats

doi:10.1128/JCM.42.8.3841-3843.2004

Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR

James R. Johnson, Krista Owens, Montse Sabate, Guillem Prats

Medicine - Veteran's Administration Medical Center

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.

Original language	English (US)
Pages (from-to)	3841-3843
Number of pages	3
Journal	Journal of clinical microbiology
Volume	42
Issue number	8
DOIs	https://doi.org/10.1128/JCM.42.8.3841-3843.2004
State	Published - Aug 2004

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abstract = "Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.",

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Rapid and specific detection of the O15:K52:H1 clonal group of Escherichia coli by gene-specific PCR

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