Rapid detection of avian pneumovirus in tissue culture by microindirect immunofluorescence test

Faris F. Jirjis, Sally L. Noll, David A. Halvorson, Kakambi V. Nagaraja, Evelyn L. Townsend, Sagar M. Goyal, Daniel P. Shaw

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

An indirect immunofluorescence (IFA) test with a 96-well, flat-bottomed microplate was developed to detect avian pneumovirus (APV) antigen in Vero cell cultures. Samples of nasal turbinates and swabs from infraorbital sinuses and trachea were collected from 4-week-old poults experimentally inoculated with APV. The APV titers by tissue culture IFA staining were compared with that of visual reading of cytopathic effect (CPE). The ability of IFA staining to detect APV antigen correlated well with visualizing CPE. The use of IFA staining of Vero cell cultures allowed detection of APV in substantially less time than the use of visualizing CPE. In addition, the use of IFA allowed specific identification of the virus in cell culture.

Original languageEnglish (US)
Pages (from-to)172-175
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume14
Issue number2
DOIs
StatePublished - Mar 2002

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