Rapid identification and characterization of antioxidants from Ligularia fischeri

Xiang Lan Piao, Xiao Yuan Mi, Yan Ze Tian, Qian Wu, Hui Shan Piao, Zhikai Zeng, Ding Wang, Xiangshu Piao

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH2Cl2, BuOH, and H2O from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2″-acetylhyperoside with potent antioxidant effects against the DPPH radical, with IC50 values of 1.31 and 7.09 μg/mL, respectively. They have not been reported from L. fischeri yet.

Original languageEnglish (US)
Pages (from-to)1689-1694
Number of pages6
JournalArchives of Pharmacal Research
Issue number12
StatePublished - Dec 1 2009


  • 2″-acetylhyperoside
  • Antioxidative activity
  • Bioassay-linked HPLC
  • Hyperoside
  • Ligularia fischeri

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