Abstract
Using a removable membrane preconcentration (mPC) cartridge, large sample volumes can be loaded prior to final assembly of the mPC capillary electrophoresis (CE) capillary. For narrow-bore (≤ 25 μm ID) uncoated mPC-CE capillaries, applied to peptide analysis, efficient moving boundary transient isotachphoresis (tITP) conditions at the onset of electrophoresis are described. The enhancement of mPC-CE-mass spectrometry (MS) technology afforded by rapid sample loading and modified moving boundary tITP conditions are demonstrated by analysis of major histocompatibility complex (MHC) class I peptides that were derived from a Kb precipitation of mouse EL-4 cells. Furthermore, we demonstrate the structural characterization of these immunologically significant molecules by mPC-CE-tandem mass spectrometry (mPC-CE-MS/MS).
Original language | English (US) |
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Pages (from-to) | 1801-1807 |
Number of pages | 7 |
Journal | ELECTROPHORESIS |
Volume | 17 |
Issue number | 12 |
DOIs | |
State | Published - 1996 |
Keywords
- Capillary electrophoresis
- Major histocompatibility complex class I peptides
- Mass spectrometry
- Preconcentration
- Rapid loading