Rapid microassays for clindamycin and gentamicin when present together and the effect of pH and of each on the antibacterial activity of the other.

L. D. Sabath, I. Toftegaard

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Abstract

Gentamicin was measured in the presence of clindamycin by using as the assay organism a strain of Staphylococcus aureus that was resistant to clindamycin; clindamycin was measured in the presence of gentamicin by using Clostridium perfringens as the assay organism. Both assays were in agar diffusion systems, were rapid (2 to 4 h), and had errors of less than 10%. Neither antibiotic antagonized or synergized with the other against a variety of organisms. The pK of clindamycin was 7.3, and the pK of gentamicin was 8.2. Gentamicin was 100 or more times as active in alkaline medium against both gram-positive and gram-negative organisms, but clindamycin showed a striking pH effect on activity only with gram-positive organisms.
Original languageUndefined/Unknown
Pages (from-to)54-59
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume6
Issue number1
StatePublished - 1974

Keywords

  • Anti-Bacterial Agents/pd [Pharmacology]
  • Clindamycin/pd [Pharmacology]
  • Clostridium perfringens/de [Drug Effects]
  • Drug Therapy, Combination/pd [Pharmacology]
  • Gentamicins/pd [Pharmacology]
  • Hydrogen-Ion Concentration
  • Microbial Sensitivity Tests

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