Magnetic resonance (MR) imaging is a powerful technique for monitoring the permeation of cryoprotective agents (CPAs) inside tissues. However, the techniques published until now suffer from inherently long imaging times, limiting the application of these techniques to slow diffusion processes and large CPA concentrations. In this study, we present a rapid MR imaging technique based on a CHESS-FLASH scheme combined with Keyhole image acquisition. This technique can image the fast permeation of Me2SO solutions into freeze-dried artificial dermal replacements for concentrations down to 10% v/v. Special attention is given to evaluating the technique for quantitative analysis. (C) 2000 Academic Press.
Bibliographical noteFunding Information:
Received October 20, 1998; accepted October 26, 1999. This research was funded in part by grants from the Minnesota Medical Foundation and the National Science Foundation (BED 9410428).
- Artificial tissue
- Rapid MR imaging