Abstract
We report a method to quantify protein levels using a combination of printed electronic and microfluidic technologies. The label-free method transduces the binding of ricin to nucleic acid aptamers into an amplified potentiometric signal. Microfluidic channels physically separate the active sensor surface from the EGT-transducer, permitting the capture of ricin in orange juice and milk. Under flow, signals arise between 1-45 minutes without any pre-processing or rinsing steps. Easy fabrication, multiplexing, and operation, combined with rapid quantitation, make our device promising for parallelized monitoring of toxin levels in food and other environmental samples.
| Original language | English (US) |
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| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1262-1263 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| State | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: Oct 9 2016 → Oct 13 2016 |
Publication series
| Name | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
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Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
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| Country/Territory | Ireland |
| City | Dublin |
| Period | 10/9/16 → 10/13/16 |
Bibliographical note
Funding Information:The authors would like to thank the NSF fellowship and Packard Fellowship for financial support. Portions of this work were performed in the Minnesota Nano Center, which receives partial support from the NSF through NNIN.
Keywords
- Biosensor
- Electrochemical
- Printed electronics
- Transistor