Rational promoter elements and evolutionary engineering approaches for efficient xylose fermentation in Saccharomyces cerevisiae

Yaxin Sang, Qian Xue, Zishang Bai, Jingyu Wang, Limin Cao

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We screened and identified a set of efficient promoters in Saccharomyces cerevisiae that maintained their relatively strong strengths to regulate the heterologous xylose-assimilating pathway genes XYL1 and XYL2, and native XKS1 and pentose phosphate pathway four genes, irrespective of glucose or xylose fermentation medium. In this study, we developed a rapid and efficient xylose-fermenting S. cerevisiae strain 7-1 based on balanced pathway expression levels driven by our proposed strong promoters. Next, 7-1 was used to initialize the evolutionary engineering, through first aerobic and anaerobic sequential batch cultivation. The finally evolved strain of 7-1E1 displayed a high ethanol yield (0.45 g/g) and low xylitol accumulation (0.13 g/g). Moreover, the evolved strain of 7-1E1 displays great potential for ethanol production from lignocellulosic biomass. This work reveals that efficient xylose assimilation is attributed to the elevated expression levels of xylose utilization genes, which was accomplished through the strong promoter rational regulation in the chromosome of the evolved strain.

Original languageEnglish (US)
Article number053104
JournalJournal of Renewable and Sustainable Energy
Volume8
Issue number5
DOIs
StatePublished - Sep 1 2016

Bibliographical note

Funding Information:
This work was financially supported by the National Natural Science Foundation of China (Grant No. 31570044). We also thank anonymous reviewers for their insightful and constructive comments during revision of this manuscript.

Publisher Copyright:
© 2016 Author(s).

Fingerprint Dive into the research topics of 'Rational promoter elements and evolutionary engineering approaches for efficient xylose fermentation in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this