Recent advances in stable isotope-enabled mass spectrometry-based plant metabolomics

Dana M. Freund, Adrian D. Hegeman

Research output: Contribution to journalReview articlepeer-review

27 Scopus citations

Abstract

Methods employing isotope labeled compounds have been an important part of the bioanalytical canon for many decades. The past fifteen years have seen the development of many new approaches using stable (non-radioactive) isotopes as labels for high-throughput bioanalytical, ‘omics-scale’ measurements of metabolites (metabolomics) and proteins (proteomics). This review examines stable isotopic labeling approaches that have been developed for labeling whole intact plants, plant tissues, or crude extracts of plant materials with stable isotopes (mainly using 2H, 13C, 15N, 18O or 34S). The application of metabolome-scale labeling for improving metabolite annotation, metabolic pathway elucidation, and relative quantification in mass spectrometry-based metabolomics of plants is also reviewed.

Original languageEnglish (US)
Pages (from-to)41-48
Number of pages8
JournalCurrent opinion in biotechnology
Volume43
DOIs
StatePublished - Feb 1 2017

Bibliographical note

Funding Information:
The authors acknowledge support from the National Science Foundation , Plant Genome Research Program, IOS-1400818 (DMF) & IOS-1238812.

Publisher Copyright:
© 2016 Elsevier Ltd

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

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