Recombinant AMP/Polypeptide Self-Assembled Monolayers with Synergistic Antimicrobial Properties for Bacterial Strains of Medical Relevance

Sergio Acosta, Luis Quintanilla, Matilde Alonso, Conrado Aparicio, José Carlos Rodríguez-Cabello

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Nosocomial infections are one of the most frequent causes of indwelling biomedical device failure. In this regard, the use of antibiofilm nanocoatings based on antimicrobial peptides (AMPs) is a promising alternative to prevent multiresistant biofilm infections. However, the limitations of chemical production impede the large-scale development of advanced antimicrobial materials that improve the properties of AMPs. Herein, we present a multifunctional modular design for the recombinant coproduction of self-assembled monolayers (SAMs) based on AMPs and elastin-like recombinamers (ELRs), which combine the antimicrobial properties of a designer AMP, GL13K, and low-fouling activity of an ELR in a synergistic manner. The inclusion of a grafting domain intended for oriented tethering onto surfaces allowed the recombinant polymers to be covalently immobilized onto model gold surfaces. The antibiofilm properties against two of the bacterial strains most frequently responsible for indwelling medical device-associated infections, namely Staphylococcus epidermidis and Staphylococcus aureus, were then evaluated. GL13K peptide was found to provide antibiofilm properties to the surface, with these being synergistically enhanced by the antifouling effect of the ELR. This new design offers a promising tool for the development of advanced AMP-based nanocoatings for medical devices with powerful and enhanced features.

Original languageEnglish (US)
Pages (from-to)4708-4716
Number of pages9
JournalACS Biomaterials Science and Engineering
Volume5
Issue number9
DOIs
StatePublished - Sep 9 2019

Bibliographical note

Funding Information:
The authors acknowledge Dr. Arturo Iban?ez and Prof. Javier Arias-Vallejo, Bioforge group, University of Valladolid, for technical assistance during the optimization of the bioproduction; Dr. Tatjana Flora, Bioforge group, University of Valladolid, for technical assistance with cytocompatibility tests. The authors are grateful for the funding from the European Commission (NMP-2014-646075), Minister of Science of the Spanish Government (PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R), Junta de Castilla y Leo?n (VA317P18), and Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y Leo?n.

Funding Information:
The authors acknowledge Dr. Arturo Ibañez and Prof. Javier Arias-Vallejo, Bioforge group, University of Valladolid, for technical assistance during the optimization of the bioproduction; Dr. Tatjana Flora, Bioforge group, University of Valladolid, for technical assistance with cytocompatibility tests. The authors are grateful for the funding from the European Commission (NMP-2014-646075), Minister of Science of the Spanish Government (PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R), Junta de Castilla y León (VA317P18), and Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León.

Publisher Copyright:
Copyright © 2019 American Chemical Society.

Keywords

  • antibiofilm
  • antimicrobial peptides
  • elastin-like recombinamers
  • recombinant
  • self-assembled monolayers

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