Reconstituting ring-rafts in bud-mimicking topography of model membranes

Yong Sang Ryu; In Ho Lee; Jeng Hun Suh; Seung Chul Park; Soojung Oh; Luke R. Jordan; Nathan J. Wittenberg; Sang Hyun Oh; Noo Li Jeon; Byoungho Lee; Atul N. Parikh; Sin Doo Lee

doi:10.1038/ncomms5507

Reconstituting ring-rafts in bud-mimicking topography of model membranes

Yong Sang Ryu, In Ho Lee, Jeng Hun Suh, Seung Chul Park, Soojung Oh, Luke R. Jordan, Nathan J. Wittenberg, Sang Hyun Oh, Noo Li Jeon, Byoungho Lee, Atul N. Parikh, Sin Doo Lee

Electrical and Computer Engineering

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein-or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and proteins from the donor membrane through restricted diffusion for the development of a ring-type raft domain of closed topology. Here, by reconstituting the bud topography in a model membrane, we demonstrate the preferential localization of cholesterol-and sphingomyelin-enriched microdomains in the collar band of the bud-neck interfaced with the donor membrane. The geometrical approach to the recapitulation of the dynamic membrane reorganization, resulting from the local radii of curvatures from nanometre-to-micrometre scales, offers important clues for understanding the active roles of the bud topography in the sorting and migration machinery of key signalling proteins involved in membrane budding.

Original language	English (US)
Article number	4507
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms5507
State	Published - Jul 24 2014

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the government of Korea (MSIP; No. 2007-0054847). Contribution by A.N.P. was supported by a grant from the U.S. Department of Energy under the award number DE-FG02-04ER46173. Contribution by L.R.J., N.J.W., S.-H.O. was supported by grants from the U.S. National Institutes of Health (NIH R01 GM092993) and the National Science Foundation (CAREER, DBI 1054191).

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abstract = "During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein-or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and proteins from the donor membrane through restricted diffusion for the development of a ring-type raft domain of closed topology. Here, by reconstituting the bud topography in a model membrane, we demonstrate the preferential localization of cholesterol-and sphingomyelin-enriched microdomains in the collar band of the bud-neck interfaced with the donor membrane. The geometrical approach to the recapitulation of the dynamic membrane reorganization, resulting from the local radii of curvatures from nanometre-to-micrometre scales, offers important clues for understanding the active roles of the bud topography in the sorting and migration machinery of key signalling proteins involved in membrane budding.",

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Reconstituting ring-rafts in bud-mimicking topography of model membranes

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