TY - JOUR
T1 - Recovery of avirulent, thermostable Newcastle disease virus strain NDV4-C from cloned cDNA and stable expression of an inserted foreign gene
AU - Zhang, Xintao
AU - Liu, Huairan
AU - Liu, Peixin
AU - Peeters, Ben P H
AU - Zhao, Chenchen
AU - Kong, Xiangang
PY - 2013/10/1
Y1 - 2013/10/1
N2 - A reverse genetics system for thermostable Newcastle disease virus (NDV) is not currently available. In this study, we developed a reverse genetics system for the avirulent and thermostable NDV4-C strain. Successful recovery of NDV4-C was achieved by using either T7 RNA polymerase or cellular RNA polymerase II to drive transcription of the full-length virus antigenome from cloned cDNA. The recovered viruses rNDV4-C (T7) and rNDV4-C (CMV) showed similar growth properties, thermostability, and virulence as the parental strain NDV4-C. The potential of rNDV4-C (T7) to serve as a viral vector was assessed by generating a recombinant virus, rNDV4-eGFP, which expressed enhanced green fluorescent protein. The rNDV4-eGFP could stably carry and express eGFP for at least fifteen passages. The reverse genetics system for NDV4-C will make it possible to analyze the genetic elements that determine thermostability and the oncolytic properties of NDV.
AB - A reverse genetics system for thermostable Newcastle disease virus (NDV) is not currently available. In this study, we developed a reverse genetics system for the avirulent and thermostable NDV4-C strain. Successful recovery of NDV4-C was achieved by using either T7 RNA polymerase or cellular RNA polymerase II to drive transcription of the full-length virus antigenome from cloned cDNA. The recovered viruses rNDV4-C (T7) and rNDV4-C (CMV) showed similar growth properties, thermostability, and virulence as the parental strain NDV4-C. The potential of rNDV4-C (T7) to serve as a viral vector was assessed by generating a recombinant virus, rNDV4-eGFP, which expressed enhanced green fluorescent protein. The rNDV4-eGFP could stably carry and express eGFP for at least fifteen passages. The reverse genetics system for NDV4-C will make it possible to analyze the genetic elements that determine thermostability and the oncolytic properties of NDV.
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U2 - 10.1007/s00705-013-1723-6
DO - 10.1007/s00705-013-1723-6
M3 - Article
C2 - 23657741
AN - SCOPUS:84884820849
SN - 0304-8608
VL - 158
SP - 2115
EP - 2120
JO - Archives of Virology
JF - Archives of Virology
IS - 10
ER -