Reduced lymphocyte infiltration during cytomegalovirus brain infection of interleukin-10deficient mice

Maxim C Cheeran, Manohar B. Mutnal, Shuxian Hu, Anibal G Armien, James R Lokensgard

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Interleukin (IL)-10 deficiency results in highly elevated levels of interferon (IFN)-, as well as the IFN-inducible chemokines CXCL9 and CXCL10 within murine cytomegalovirus (MCMV)-infected brains. To test the hypothesis that these elevated chemokine levels would result in enhanced brain infiltration, we compared immune cell infiltration in response to MCMV brain infection between wild-type and IL-10 knockout (KO) mice. Longitudinal analysis following adoptive transfer of cells from -actinluciferase transgenic wild-type mice showed maximal brain infiltration by peripheral immune cells occurred at 5 days post infection. Although the overall percentage of CD45(hi) cells infiltrating the brain was not altered by IL-10 deficiency, paradoxically, despite elevated chemokine levels, reduced T lymphocyte (CD8 + ) and natural killer (NK) (CD49b + ) cell infiltration into the brain was observed in IL-10deficient animals. This decreased lymphocyte infiltration was associated with elevated levels of the lymph node homing receptor L-selectin/CD62L on CD8+ T cells. Lymph node cells obtained from MCMV-infected mice deficient in IL-10 also displayed reduced migration towards CXCL10 when compared to wild-type animals. Taken together, these data show that despite elevated chemokine levels, absence of IL-10 results in reduced lymphocyte infiltration into MCMV-infected brains.

Original languageEnglish (US)
Pages (from-to)334-342
Number of pages9
JournalJournal of neurovirology
Volume15
Issue number4
DOIs
StatePublished - 2009

Keywords

  • Brain
  • IL-10
  • Lymphocytes
  • MCMV

Fingerprint Dive into the research topics of 'Reduced lymphocyte infiltration during cytomegalovirus brain infection of interleukin-10deficient mice'. Together they form a unique fingerprint.

Cite this