Reduction of Multidrug-Resistant Salmonella enterica Serovar Heidelberg Using a Dairy-Originated Probiotic Bacterium, Propionibacterium freudenreichii freudenreichii B3523, in Growing Turkeys

D. V.T. Nair, J. Vazhakkattu Thomas, G. Dewi, S. Noll, J. Brannon, A. Kollanoor Johny

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We investigated the antibacterial efficacy of a dairy-origin probiotic bacterium, Propionibacterium freudenreichii freudenreichii B3523 (PF), on a multidrug-resistant strain of Salmonella enterica serovar Heidelberg (SH) in growing turkeys. In 2 independent experiments, day-old poults (N = 24/experiment) were randomly assigned to 3 treatment groups (8 turkeys/group). The groups were: a negative control (NC; turkeys without PF supplementation or SH challenge), an SH control (SC; turkeys challenged with SH without PF supplementation), and a test group (PFS; turkeys supplemented with PF and challenged with SH) raised until 7 wk of age. Turkeys in the PFS group received PF once in 2 d at 1012 cfu in 5 gallon drinking water from day 0 until 6 wk of age. Turkeys in SC and PFS groups were challenged with SH as crop gavage at 6 wk of age. After SH inoculation, the PFS group received PF daily for 7 d until the end of the study. The colonization of SH and PF in turkey ceca was determined 2 and 7 d post-inoculation of the pathogen. In addition, the effect of PF supplementation on the dissemination of SH to liver and spleen was determined. Results indicated that PF was recoverable at 4 to 4.5 log10 cfu/g at the end of the study. Additionally, PF supplementation significantly reduced SH colonization in the PFS group by 1 to 1.3 log10 cfu/g in the turkey ceca 2 and 7 d after SH inoculation (P ≤ 0.05), compared to the SC group. In addition, PF supplementation reduced dissemination of SH to liver and spleen (P ≤ 0.05), compared to the SC group. Results indicate that PF could be a potential probiotic strategy in turkeys to control SH colonization at a later stage of turkey production.

Original languageEnglish (US)
Pages (from-to)356-363
Number of pages8
JournalJournal of Applied Poultry Research
Volume28
Issue number2
DOIs
StatePublished - Jun 1 2019

Bibliographical note

Funding Information:
The authors would like to thank Tim Johnson, PhD, in the Veterinary Population Medicine department and Ryan Cox, PhD, in the Animal Science department for the review of this chapter in the primary author's doctoral thesis.

Keywords

  • Propionibacterium freudenreichii
  • Salmonella Heidelberg
  • antibiotic alternative
  • multidrug-resistant
  • probiotics
  • turkeys

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