The efficacies of trans-cinnamaldehyde (TC) and eugenol (EG) for reducing Salmonella enterica serovar Enteritidis colonization in broiler chickens were investigated. In three experiments for each compound, 1-day-old chicks (n=75/experiment) were randomly assigned to five treatment groups (n=15/treatment group): negative control (-ve S. Enteritidis,-ve TC, or EG), compound control (-ve S. Enteritidis,+ve 0.75% [vol/wt] TC or 1% [vol/wt] EG), positive control (+ve S. Enteritidis,-ve TC, or EG), low-dose treatment (+ve S. Enteritidis,+ve 0.5% TC, or 0.75% EG), and high-dose treatment (+ve S. Enteritidis,+ve 0.75% TC, or 1% EG). On day 0, birds were tested for the presence of any inherent Salmonella (n=5/experiment). On day 8, birds were inoculated with ~8.0 log10 CFU S. Enteritidis, and cecal colonization by S. Enteritidis was ascertained (n=10chicks/experiment) after 24h (day 9). Six birds from each treatment group were euthanized on days 7 and 10 after inoculation, and cecal S. Enteritidis numbers were determined. TC at 0.5 or 0.75% and EG at 0.75 or 1% consistently reduced (P<0.05) S. Enteritidis in the cecum (≥3 log10CFU/g) after 10 days of infection in all experiments. Feed intake and body weight were not different for TC treatments (P>0.05); however, EG supplementation led to significantly lower (P<0.05) body weights. Follow-up in vitro experiments revealed that the subinhibitory concentrations (SICs, the concentrations that did not inhibit Salmonella growth) of TC and EG reduced the motility and invasive abilities of S. Enteritidis and downregulated expression of the motility genes flhC and motA and invasion genes hilA, hilD, and invF. The results suggest that supplementation with TC and EG through feed can reduce S. Enteritidis colonization in chickens.