Regulation of apoptotic pathways in bovine γ/δ T cells

Mingqi Deng, Jin Liu, Colleen N. Pelak, Cheryl A. Lancto, Mitchell S. Abrahamsen

Research output: Contribution to journalArticlepeer-review

Abstract

T lymphocytes bearing γ/δ TCRs are a major population of T cells in neonatal calves and discrete subsets of γ/δ T cells display tissue-specific accumulation and responsiveness to infection. To enhance our understanding of the immunobiology of γ/δ T cells, we characterized the gene expression profile of circulating bovine γ/δ T cells following stimulation with recombinant human IL-2 and ConA. Statistical analysis of microarray data identified 108 genes with significantly altered expression, including four genes associated with apoptosis. Real-time reverse transcription-PCR (RT-PCR) analysis of 15 genes related to apoptotic pathways showed that both the Fas-mediated and the mitochondrial apoptotic pathways were repressed in circulating bovine γ/δ T cells in response to mitogen activation, indicating that stimulated peripheral bovine γ/δ T cells are resistant to activation-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)15-23
Number of pages9
JournalVeterinary immunology and immunopathology
Volume105
Issue number1-2
DOIs
StatePublished - May 1 2005

Bibliographical note

Funding Information:
This work was supported in part by grants 99-35205-8618 and 00-52100-9612 from the United States Department of Agriculture.

Keywords

  • Apoptosis
  • Microarray
  • Mitogenic stimulation
  • Real-time PCR
  • γ/δ T cell

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