Relative Contributions of Host and Microbial Factors in Bacterial Translocation

Carol L. Wells, Robert P. Jechorek, Kristen J. Gillingham

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

To study the relative contributions of host and microbial factors in bacterial translocation, germfree mice were mono-associated with either Proteus mirabilis, Escherichia coli, or Enterococcus faecalis. Germfree mice included T-cell–deficient nude mice and normal littermates, natural killer cell–deficient beige mice and normal littermates, and triply immunodeficient mice with beige, T-cell, and B-cell mutations and their littermates. Each bacterial species colonized the cecum in similarly high numbers. Bacteria were recovered from the mesenteric lymph node of every mouse in inconsistent numbers, eg, greater numbers of P mirabilis and E coli were recovered from T-cell–deficient nude mice than from their normal littermates, but the opposite was observed with E faecalis. Comparing the three bacterial species resulted in relatively consistent observations, eg, the incidence of E faecalis translocation to the liver was greater than that of E coli or P mirabilis translocation. Thus, the identity of the translocating microbe significantly affected the recovery of viable translocating bacteria.

Original languageEnglish (US)
Pages (from-to)247-252
Number of pages6
JournalArchives of Surgery
Volume126
Issue number2
DOIs
StatePublished - Feb 1991

Fingerprint Dive into the research topics of 'Relative Contributions of Host and Microbial Factors in Bacterial Translocation'. Together they form a unique fingerprint.

Cite this