Abstract
Introduction: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. Materials and methods: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC (%VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. Results: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 %VCAM-1, p = 0.592) between baseline and 1 week follow-up. Conclusion: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.
Original language | English (US) |
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Pages (from-to) | 463-471 |
Number of pages | 9 |
Journal | Biomarkers in Medicine |
Volume | 10 |
Issue number | 5 |
DOIs | |
State | Published - May 2016 |
Bibliographical note
Publisher Copyright:© 2016 Future Medicine Ltd.
Keywords
- biomarkers
- cardiovascular disease risk
- circulating endothelial cells
- obesity
- pediatric
- reproducibility
- youth