Reproducibility of endothelial microparticles in children and adolescents

Elise F. Northrop, Liming C. Milbauer, Kyle D. Rudser, Claudia K. Fox, Anna N. Solovey, Alexander M. Kaizer, Robert P. Hebbel, Aaron S. Kelly, Justin R. Ryder

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: We assessed reproducibility of endothelial microparticles (EMPs) enumeration among youth. Methods & results: Four microparticle (MP) indices - total MP per microliter platelet free plasma (PFP), total EMPs per microliter PFP, percent activated EMPs and percent lactadherin positive (LACT[+]) of total EMPs - were measured at two visits (baseline and 7 ± 3 days follow-up) to determine reproducibility overall and by obesity status. We examined CD31+ or CD144+ with CD41-EMP events of size 0.3-1.0 μm. No statistically significant differences were observed between visits for any of the four MP indices. The within-participant and between-participant coefficient of variation was acceptable (range: 1.13-2.37) with good intraclass-correlation coefficient for all indices except total MP per microliter (range: 0.10-1.00). Conclusion: Total EMPs per microliter PFP, percent-activated EMPs and percent LACT(+) of total EMPs are reproducible among youth.

Original languageEnglish (US)
Pages (from-to)43-51
Number of pages9
JournalBiomarkers in Medicine
Volume14
Issue number1
DOIs
StatePublished - 2019

Bibliographical note

Funding Information:
AS Kelly has served on pediatric obesity advisory boards (Novo Nordisk; Orexigen and Vivus) and serves as the signatory author for a pediatric obesity clinical trial sponsored by Novo Nordisk but does not receive personal or professional income for his services. AS Kelly receives research support (drug/placebo) from Astra Zeneca. JR Ryder receives research support (drug/placebo) from Boehringer Ingelheim. This study was supported by the NIH (grant number: 5R01HL110957). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

Publisher Copyright:
© 2019 Future Medicine Ltd.

Keywords

  • biomarkers
  • endothelial
  • microparticles
  • obesity
  • reproducibility
  • youth

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