Repulsive DNA-DNA interactions accelerate viral DNA packaging in phage Phi29

Nicholas Keller, Damian Deltoro, Shelley Grimes, Paul J. Jardine, Douglas E. Smith

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We use optical tweezers to study the effect of attractive versus repulsive DNA-DNA interactions on motor-driven viral packaging. Screening of repulsive interactions accelerates packaging, but induction of attractive interactions by spermidine3+ causes heterogeneous dynamics. Acceleration is observed in a fraction of complexes, but most exhibit slowing and stalling, suggesting that attractive interactions promote nonequilibrium DNA conformations that impede the motor. Thus, repulsive interactions facilitate packaging despite increasing the energy of the theoretical optimum spooled DNA conformation.

Original languageEnglish (US)
Article number248101
JournalPhysical review letters
Volume112
Issue number24
DOIs
StatePublished - Jun 17 2014

Bibliographical note

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

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