We retrospectively analyzed five Candida albicans isolates from two infection episodes in a single patient 6 months apart. Using contour-clamped homogeneous field electrophoresis (CHEF), random amplified polymorphic DNA (RAPD) fingerprinting, and restriction fragment length polymorphism (RFLP) complex probe 27A as means of molecular typing, we demonstrate an unvarying genotype amongst the infection-causing C. albicans strains. Several months later, the patient yielded C. glabrata in a yeast survey of oral and rectal sites. The preponderance of C. glabrata and lack of C. albicans isolated from normal flora sites suggests that this patient harbored the prior C. albicans bloodstream isolate on a Gore-Tex graft for 6 months prior to the second episode of fungemia.
Bibliographical noteFunding Information:
This work was supported, in part, by Grant AI-01411 from the National Institutes of Health. We thank Paul T. Magee for critical review of this manuscript, Anja Forche for optimized DNA fingerprinting protocols and Brendan Cormack for the C. glabrata BG2 strain.
- Candida albicans
- Pulsed field gel electrophoresis