Reservoir of Candida albicans infection in a vascular bypass graft demonstrates a stable karyotype over six months

Paul Lephart; Patricia Ferrieri; Jo Anne H. van Burik

doi:10.1080/13693780310001644662

Reservoir of Candida albicans infection in a vascular bypass graft demonstrates a stable karyotype over six months

Paul Lephart, Patricia Ferrieri, Jo Anne H. van Burik

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7 Scopus citations

Abstract

We retrospectively analyzed five Candida albicans isolates from two infection episodes in a single patient 6 months apart. Using contour-clamped homogeneous field electrophoresis (CHEF), random amplified polymorphic DNA (RAPD) fingerprinting, and restriction fragment length polymorphism (RFLP) complex probe 27A as means of molecular typing, we demonstrate an unvarying genotype amongst the infection-causing C. albicans strains. Several months later, the patient yielded C. glabrata in a yeast survey of oral and rectal sites. The preponderance of C. glabrata and lack of C. albicans isolated from normal flora sites suggests that this patient harbored the prior C. albicans bloodstream isolate on a Gore-Tex graft for 6 months prior to the second episode of fungemia.

Original language	English (US)
Pages (from-to)	255-260
Number of pages	6
Journal	Medical mycology
Volume	42
Issue number	3
DOIs	https://doi.org/10.1080/13693780310001644662
State	Published - Jun 2004

Bibliographical note

Funding Information:
This work was supported, in part, by Grant AI-01411 from the National Institutes of Health. We thank Paul T. Magee for critical review of this manuscript, Anja Forche for optimized DNA fingerprinting protocols and Brendan Cormack for the C. glabrata BG2 strain.

Keywords

Candida albicans
Karyotype
Pulsed field gel electrophoresis

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title = "Reservoir of Candida albicans infection in a vascular bypass graft demonstrates a stable karyotype over six months",

abstract = "We retrospectively analyzed five Candida albicans isolates from two infection episodes in a single patient 6 months apart. Using contour-clamped homogeneous field electrophoresis (CHEF), random amplified polymorphic DNA (RAPD) fingerprinting, and restriction fragment length polymorphism (RFLP) complex probe 27A as means of molecular typing, we demonstrate an unvarying genotype amongst the infection-causing C. albicans strains. Several months later, the patient yielded C. glabrata in a yeast survey of oral and rectal sites. The preponderance of C. glabrata and lack of C. albicans isolated from normal flora sites suggests that this patient harbored the prior C. albicans bloodstream isolate on a Gore-Tex graft for 6 months prior to the second episode of fungemia.",

keywords = "Candida albicans, Karyotype, Pulsed field gel electrophoresis",

author = "Paul Lephart and Patricia Ferrieri and {van Burik}, {Jo Anne H.}",

note = "Funding Information: This work was supported, in part, by Grant AI-01411 from the National Institutes of Health. We thank Paul T. Magee for critical review of this manuscript, Anja Forche for optimized DNA fingerprinting protocols and Brendan Cormack for the C. glabrata BG2 strain.",

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AU - Ferrieri, Patricia

AU - van Burik, Jo Anne H.

N1 - Funding Information: This work was supported, in part, by Grant AI-01411 from the National Institutes of Health. We thank Paul T. Magee for critical review of this manuscript, Anja Forche for optimized DNA fingerprinting protocols and Brendan Cormack for the C. glabrata BG2 strain.

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N2 - We retrospectively analyzed five Candida albicans isolates from two infection episodes in a single patient 6 months apart. Using contour-clamped homogeneous field electrophoresis (CHEF), random amplified polymorphic DNA (RAPD) fingerprinting, and restriction fragment length polymorphism (RFLP) complex probe 27A as means of molecular typing, we demonstrate an unvarying genotype amongst the infection-causing C. albicans strains. Several months later, the patient yielded C. glabrata in a yeast survey of oral and rectal sites. The preponderance of C. glabrata and lack of C. albicans isolated from normal flora sites suggests that this patient harbored the prior C. albicans bloodstream isolate on a Gore-Tex graft for 6 months prior to the second episode of fungemia.

AB - We retrospectively analyzed five Candida albicans isolates from two infection episodes in a single patient 6 months apart. Using contour-clamped homogeneous field electrophoresis (CHEF), random amplified polymorphic DNA (RAPD) fingerprinting, and restriction fragment length polymorphism (RFLP) complex probe 27A as means of molecular typing, we demonstrate an unvarying genotype amongst the infection-causing C. albicans strains. Several months later, the patient yielded C. glabrata in a yeast survey of oral and rectal sites. The preponderance of C. glabrata and lack of C. albicans isolated from normal flora sites suggests that this patient harbored the prior C. albicans bloodstream isolate on a Gore-Tex graft for 6 months prior to the second episode of fungemia.

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KW - Pulsed field gel electrophoresis

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ER -

Reservoir of Candida albicans infection in a vascular bypass graft demonstrates a stable karyotype over six months

Abstract

Bibliographical note

Keywords

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