Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.
Bibliographical noteFunding Information:
We are grateful to the germplasm banks at Kansas State University, International Center for Agricultural Research in the Dry Areas, USDA-ARS National Small Grains Collection, and the N. I. Vavilov Research Institute of Plant Industry, and to our colleagues J. Raupp, J. Dvorak, and C. Hiebert for providing seed of Ae. tauschii. We thank our colleagues Y. Yue and JIC Horticultural Services for plant husbandry, J. Brown for helpful discussions, M. Ambrose and A. Meldrum for help with MTAs, K. Witek for technical assistance with RenSeq, M. Rouse and Y. Jin for PGT isolates, P. Solomon for help with bait source sequences, the International Wheat Sequencing Consortium for pre-publication access to REFSEQ v1.0, and B. McIntosh for critical reading of the manuscript. This research was supported by the NBI Computing Infrastructure for Science (CiS) group and financed by the Two Blades Foundation, USA to B.J.S. and B.B.H.W.; the Biotechnology and Biological Sciences Research Council (BBSRC) to B.B.H.W.; the BBSRC Designing Future Wheat Cross-Institute Strategic Programme to A.R.B. and B.B.H.W.; the Norwich Research Park Translational Fund to B.B.H.W.; the Lieberman-Okinow Endowment at the University of Minnesota to B.J.S.; the Grains Research and Development Corporation, Australia to E.L. and H.B.; the Gordon and Betty Moore Foundation to J.D.G.J.; and in-kind support by Arbor Biosciences.
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