The objective of this work was to optimise different parameters (solvent concentration, time and temperature) for antioxidant extraction from kinema, demonstrated by total phenol content (TPC) and DPPH-scavenging activity (DSA), using response surface methodology. A central composite design was performed to determine the effect of solvent concentration (methanol, 30-100%), temperature (30-60 °C) and time (30-150 min) on the TPC and DSA of the extract. The solvent concentration and temperature had the most significant (p < 0.05) effect. The optimum conditions for the TPC extraction and DSA were 100% methanol, 50 °C and 30 min, in which 140 mg of gallic acid equivalent (GAE) g-1 lyophilised extract and 52% DSA were predicted, while 135 mg GAE g-1 lyophilised extract and 56% DSA were experimentally obtained. No significant difference (p < 0.05) was found between the experimental and predicted values of the response variables.
Bibliographical noteFunding Information:
We thankfully acknowledge the financial support provided by the University of North Bengal for carrying out this work.
- Antioxidant extraction
- Central composite design
- DPPH-scavenging activity
- Total phenol content