TY - JOUR
T1 - Retrovirus-mediated transfer of viral IL-10 gene prolongs murine cardiac allograft survival
AU - Qin, Lihui
AU - Chavin, Kenneth D.
AU - Ding, Yaozhong
AU - Tahara, Hideaki
AU - Favaro, Justin P.
AU - Woodward, Jennifer E.
AU - Suzuki, Tadamichi
AU - Robbins, Paul D.
AU - Lotze, Michael T.
AU - Bromberg, Jonathan S.
PY - 1996/3/15
Y1 - 1996/3/15
N2 - A murine heterotopic, nonvascularized cardiac allograft model was used to examine the effects of the immunosuppressive cytokine, viral IL-10 (vIL-10), delivered by gene transfer on graft rejection. Retroviral-mediated gene transfer and expression of vIL-10 significantly prolonged allograft survival, without conventional systemic immunosuppression, from 12.1 ± 0.8 days to 39.4 ± 2.5 days (p < 0.0001). The effect was specific, dose dependent, and restricted to the site of transplantation. PCR analysis demonstrated specific expression of the transferred gene within the allograft. Analysis of the cellular infiltrate in the allografts showed a reduction in T cells and alloantigen-specific cytotoxic T cells and IL-2-producing helper T cells. Thus, the transient local expression of a gene encoding an immunosuppressive protein within a graft can generate local immunosuppression, making gene therapy a viable approach for facilitating transplantation.
AB - A murine heterotopic, nonvascularized cardiac allograft model was used to examine the effects of the immunosuppressive cytokine, viral IL-10 (vIL-10), delivered by gene transfer on graft rejection. Retroviral-mediated gene transfer and expression of vIL-10 significantly prolonged allograft survival, without conventional systemic immunosuppression, from 12.1 ± 0.8 days to 39.4 ± 2.5 days (p < 0.0001). The effect was specific, dose dependent, and restricted to the site of transplantation. PCR analysis demonstrated specific expression of the transferred gene within the allograft. Analysis of the cellular infiltrate in the allografts showed a reduction in T cells and alloantigen-specific cytotoxic T cells and IL-2-producing helper T cells. Thus, the transient local expression of a gene encoding an immunosuppressive protein within a graft can generate local immunosuppression, making gene therapy a viable approach for facilitating transplantation.
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M3 - Article
C2 - 8690923
AN - SCOPUS:13344249764
SN - 0022-1767
VL - 156
SP - 2316
EP - 2323
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -