Reversed-phase high-performance liquid chromatographic separation and quantification of lantadenes using isocratic systems

Om Prakash Sharma, Sarita Sharma, Rajinder Kumar Dawra

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Isocratic systems of varying suitability are reported for reversed- phase HPLC of lantadenes, the pentacyclic triterpenoids from the hepatotoxic plant Lantana camara var. aculeata. The column used was a Nova-Pak C18 (250x4.6 mm). The detection was at 210 nm and 240 nm. The mobile phase acetonitrile-water-acetic acid (80:20:0.01) provided baseline separation of all the lantadenes except lantadenes A and C. The mobile phase methanol- water-acetic acid (85:15:0.01) also provided resolution of all the lantadenes but the retention time windows were very broad. The mobile phase methanol- acetonitrile-water-acetic acid (68:20:12:0.01) has been found to be the most suitable for the separation and quantitation of lantadenes.

Original languageEnglish (US)
Pages (from-to)181-184
Number of pages4
JournalJournal of Chromatography A
Volume786
Issue number1
DOIs
StatePublished - Oct 24 1997

Bibliographical note

Funding Information:
This work has been supported by a research grant from the Department of Science and Technology, New Delhi, India. S.S. thanks the DST for the award of a junior research fellowship. The authors are grateful to Dr. Kiran Singh, Director of the Institute for the facilities and to Dr. B. Singh, Scientist-in-charge for helpful discussions.

Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.

Keywords

  • Lantadenes
  • Lantana camara
  • Triterpenoids

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