Reversible Re-programing of Cell-Cell Interactions

Kari Gabrielse, Amit Gangar, Nigam Kumar, Jae Chul Lee, Adrian Fegan, Jing Jing Shen, Qing Li, Daniel Vallera, Carston R. Wagner

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The ability to engineer and re-program the surfaces of cells would provide an enabling synthetic biological method for the design of cell- and tissue-based therapies. A new cell surface-engineering strategy is described that uses lipid-chemically self-assembled nanorings (lipid-CSANs) that can be used for the stable and reversible modification of any cell surface with a molecular reporter or targeting ligand. In the presence of a non-toxic FDA-approved drug, the nanorings were quickly disassembled and the cell-cell interactions reversed. Similar to T-cells genetically engineered to express chimeric antigen receptors (CARS), when activated peripheral blood mononuclear cells (PBMCs) were functionalized with the anti-EpCAM-lipid-CSANs, they were shown to selectively kill antigen-positive cancer cells. Taken together, these results demonstrate that lipid-CSANs have the potential to be a rapid, stable, and general method for the reversible engineering of cell surfaces and cell-cell interactions. Lipid chemically self-assembled nanorings (lipid-CSANs) can be used for the stable and reversible modification of any cell surface with a molecular reporter or targeting ligand. In the presence of the antibacterial drug trimethoprim the nanorings were quickly disassembled and the cell-cell interactions reversed.

Original languageEnglish (US)
Pages (from-to)5112-5116
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number20
DOIs
StatePublished - May 12 2014

Keywords

  • cell-cell interactions
  • chimeric antigen receptors
  • nanoparticles
  • oligomerization
  • self-assembly

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