Abstract
The ability to engineer and re-program the surfaces of cells would provide an enabling synthetic biological method for the design of cell- and tissue-based therapies. A new cell surface-engineering strategy is described that uses lipid-chemically self-assembled nanorings (lipid-CSANs) that can be used for the stable and reversible modification of any cell surface with a molecular reporter or targeting ligand. In the presence of a non-toxic FDA-approved drug, the nanorings were quickly disassembled and the cell-cell interactions reversed. Similar to T-cells genetically engineered to express chimeric antigen receptors (CARS), when activated peripheral blood mononuclear cells (PBMCs) were functionalized with the anti-EpCAM-lipid-CSANs, they were shown to selectively kill antigen-positive cancer cells. Taken together, these results demonstrate that lipid-CSANs have the potential to be a rapid, stable, and general method for the reversible engineering of cell surfaces and cell-cell interactions. Lipid chemically self-assembled nanorings (lipid-CSANs) can be used for the stable and reversible modification of any cell surface with a molecular reporter or targeting ligand. In the presence of the antibacterial drug trimethoprim the nanorings were quickly disassembled and the cell-cell interactions reversed.
Original language | English (US) |
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Pages (from-to) | 5112-5116 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 53 |
Issue number | 20 |
DOIs | |
State | Published - May 12 2014 |
Keywords
- cell-cell interactions
- chimeric antigen receptors
- nanoparticles
- oligomerization
- self-assembly