Review of the application of T-matrix calculations for determining the structure of cell nuclei with angle-resolved light scattering measurements

Michael G. Giacomelli, Kevin J. Chalut, Julie H. Ostrander, Adam Wax

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Biomedical applications of light scattering have traditionally relied on Mie theory as a theoretical basis for analyzing experimental measurements. While this approach is computationally accessible, the use of Mie theory cannot always account for the spheroidal nature of biological scatterers such as cell nuclei. As an alternative, we have developed light scattering codes based on the T-matrix formalism to analyze angle-resolved light scattering measurements. In this paper, we discuss the development of computer codes to create a database of scattering profiles, validation of the database using experimental measurements of tissue phantoms containing spheroidal scatterers, and recent applications of the approach to analyze the structure of cell nuclei. The reviewed studies show that while the T-matrix approach adds important information for some light scattering studies, the additional computational cost may not always be justified when compared to the Mie theory.

Original languageEnglish (US)
Article number5280380
Pages (from-to)900-908
Number of pages9
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume16
Issue number4
DOIs
StatePublished - Jul 2010

Bibliographical note

Funding Information:
Manuscript received August 2, 2009; revised August 26, 2009; accepted August 27, 2009. Date of publication October 6, 2009; date of current version August 6, 2010. This work was supported by the National Institutes of Health under Grant NCI R33-CA109907 and Grant NCI R21CA120128, by the National Science Foundation under Grant BES 03-48204, and by the Department of Defense under Grant BC073290.

Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.

Keywords

  • Biomedical imaging
  • Mie theory
  • T-matrix
  • light scattering

Fingerprint

Dive into the research topics of 'Review of the application of T-matrix calculations for determining the structure of cell nuclei with angle-resolved light scattering measurements'. Together they form a unique fingerprint.

Cite this