Revisionist look at solvophobic driving forces in reversed-phase liquid chromatography. II. Partitioning vs. adsorption mechanism in monomeric alkyl bonded phase supports

Lay Choo Tan, Peter W. Carr

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

The partitioning and absorption mechanisms of retention in reversed-phase liquid chromtography (RPLC) were examined based on the free energy of transfer of a methylene group from organic aqueous mixtures to bulk hexadecane. Retention on monomeric bonded phases with octyl chains or longer are dominated by a partitioning-like mechanism. An adsorption-like mechanism contributes to retention in monomeric bonded phases with short bonded chains or with low surface coverage density. The retention mechanism becomes more adsorption-like as the bonded chains decrease.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalJournal of Chromatography A
Volume775
Issue number1-2
DOIs
StatePublished - Jul 18 1997

Keywords

  • Adsorption
  • Partitioning
  • Retention mechanisms
  • Solvophobic theory
  • Stationary phases, LC

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