RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata L. Walp)

G. O. Myers, C. A. Fatokun, N. D. Young

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33 Scopus citations

Abstract

Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation of associations between genes of interest and RFLP markers. A cross between an aphid (Aphis craccivora Koch) resistant cultivated cowpea, IT84S-2246-4, and an aphid susceptible wild cowpea, NI 963, was screened for both aphid phenotype and RFLP marker segregation. One RFLP marker, bg4D9b, was found to be tightly linked to the aphid resistance gene (Rac1) and several flanking markers in the same linkage group (linkage group 1) were also identified. The close association of Rac1 and RFLP bg4D9b presents a real potential for cloning this insect resistance gene.

Original languageEnglish (US)
Pages (from-to)181-187
Number of pages7
JournalEuphytica
Volume91
Issue number2
StatePublished - Nov 26 1996

Keywords

  • Aphis craccivora
  • DNA markers
  • Vigna unguiculata
  • cowpea
  • insect
  • legume
  • restriction fragment length polymorphisms

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