RhBMP-2 modulation of gene expression in infected segmental bone defects

Katherine E. Brick, Xinqian Chen, Jamie Lohr, Andrew H. Schmidt, Louis S. Kidder, William D. Lew

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The osteoinductive capability of BMPs appears diminished in the setting of acute infection. We applied rhBMP-2 to a segmental defect in a rat femur and measured the expression of key bone formation genes in the presence of acute infection. Types I and II collagen, osteocalcin, and BMP Type II receptor mRNA expression were characterized in 72 Sprague-Dawley rats, which received either bovine collagen carrier with 200 μg rhBMP-2 plus Staphylococcus aureus, carrier with bacteria only, carrier with rhBMP-2 only, or carrier alone. Six animals from each group were euthanized at 1, 2, and 4 weeks. Total RNA was isolated and extracted, and mRNA was determined by real-time comparative quantitative PCR. Infected defects had little expression of collagen I and II and osteocalcin mRNAs, while BMP receptor II expression with infection was greater than carrier-only controls at Weeks 2 and 4. Notably, all four genes were upregulated in infected defects in the presence of rhBMP-2. Thus, in a clinical setting with a high risk of infection and nonunion, such as a compound fracture with bone loss, rhBMP-2 may increase the rate and extent of bone formation. Even if infection does occur, rhBMP-2 may allow a quicker overall recovery time.

Original languageEnglish (US)
Pages (from-to)3096-3103
Number of pages8
JournalClinical orthopaedics and related research
Volume467
Issue number12
DOIs
StatePublished - Dec 2009

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