TY - JOUR
T1 - Rhizosphere bacterial communities associated with long-lived perennial prairie plants vary in diversity, composition, and structure
AU - Rosenzweig, N.
AU - Bradeen, J. M.
AU - Tu, Z. J.
AU - McKay, S. J.
AU - Kinkel, L. L.
PY - 2013
Y1 - 2013
N2 - The goal of this research was to investigate the variation in rhizosphere microbial community composition, diversity, and structure among individual Andropogon gerardii Vitman (big bluestem) and Lespedeza capitata Michx. (bush clover). Bacterial communities from the rhizosphere of 10 plants of each species (n = 20 plants total) were explored using a culture-independent pipeline. Microbial communities associated with both host plants had high bacterial diversity within individual plant rhizosphere and taxa unique to individual rhizospheres. Bacterial communities associated with the rhizosphere of A. gerardii were consistently more diverse than those associated with L. capitata, and there were significant differences between plant species in rhizosphere bacterial community composition. Differences included microbial taxa with no known functional relationship with their preferred host species, including sulfide-methylating obligate anaerobes (Holophaga), complete denitrifiers (Rhodoplanes), sludge inhabitants (Ktedonobacter), and nitrate oxidizers (Nitrospira). These results suggest the potential for plant species to have significant impacts on a broad array of ecosystem functions (e.g., cycling of carbon, nitrogen sulfurs, metals, and trace elements) via their selective impacts on soil microbes. However, sequence-based community analysis and the corresponding lack of intact microbial cultures limits understanding of the potential influences of enriched microbial taxa on plant hosts and their roles in ecosystem functioning.
AB - The goal of this research was to investigate the variation in rhizosphere microbial community composition, diversity, and structure among individual Andropogon gerardii Vitman (big bluestem) and Lespedeza capitata Michx. (bush clover). Bacterial communities from the rhizosphere of 10 plants of each species (n = 20 plants total) were explored using a culture-independent pipeline. Microbial communities associated with both host plants had high bacterial diversity within individual plant rhizosphere and taxa unique to individual rhizospheres. Bacterial communities associated with the rhizosphere of A. gerardii were consistently more diverse than those associated with L. capitata, and there were significant differences between plant species in rhizosphere bacterial community composition. Differences included microbial taxa with no known functional relationship with their preferred host species, including sulfide-methylating obligate anaerobes (Holophaga), complete denitrifiers (Rhodoplanes), sludge inhabitants (Ktedonobacter), and nitrate oxidizers (Nitrospira). These results suggest the potential for plant species to have significant impacts on a broad array of ecosystem functions (e.g., cycling of carbon, nitrogen sulfurs, metals, and trace elements) via their selective impacts on soil microbes. However, sequence-based community analysis and the corresponding lack of intact microbial cultures limits understanding of the potential influences of enriched microbial taxa on plant hosts and their roles in ecosystem functioning.
KW - Andropogon gerardii
KW - Lespedeza capitata
KW - Microbial communities
KW - Pyrosequencing
KW - rDNA 16S gene V3 region
UR - http://www.scopus.com/inward/record.url?scp=84879924551&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879924551&partnerID=8YFLogxK
U2 - 10.1139/cjm-2012-0661
DO - 10.1139/cjm-2012-0661
M3 - Article
C2 - 23826959
AN - SCOPUS:84879924551
SN - 0008-4166
VL - 59
SP - 494
EP - 502
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
IS - 7
ER -