RNAi screening in Spodoptera frugiperda

Subhanita Ghosh, Gatikrushna Singh, Bindiya Sachdev, Ajit Kumar, Pawan Malhotra, Sunil K. Mukherjee, Raj K. Bhatnagar

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

RNA interference is a potent and precise reverse genetic approach to carryout large-scale functional genomic studies in a given organism. During the past decade, RNAi has also emerged as an important investigative tool to understand the process of viral pathogenesis. Our laboratory has successfully generated transgenic reporter and RNAi sensor line of Spodoptera frugiperda (Sf21) cells and developed a reversal of silencing assay via siRNA or shRNA guided screening to investigate RNAi factors or viral pathogenic factors with extraordinary fi delity. Here we describe empirical approaches and conceptual understanding to execute successful RNAi screening in Spodoptera frugiperda 21-cell line.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-212
Number of pages14
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1470
ISSN (Print)1064-3745

Keywords

  • Insect RNAi
  • RNA interference
  • RNAi screening
  • Sf21 cells
  • SiRNA screening
  • Spodoptera frugiperda

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