Role of endothelial cells in human hematopoiesis: Modulation of mixed colony growth in vitro

J. L. Ascensao, G. M. Vercellotti, Harry S Jacob, E. D. Zanjani

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The identification of clonal human multipotent hematopoietic progenitors has permitted an analysis of the growth factor requirements for these cells. Human endothelial cell cultures were used to examine the effects of media conditioned by the endothelial cells on human multipotent (CFU-mix) and committed erythroid (BFU-E, CFU-E) and myeloid (CFU-GM) precursors. These studies demonstrate that endothelial cells produce proteins of approximately 30,000 daltons, with isoelectric focusing points of 4.5 and 7.2, which stimulate the growth of human BFU-E and CFU-mix. A heat-labile protein(s) of 30,000 and 15,000 daltons stimulated the proliferation and differentiation of granulocyte-macrophage (CFU-GM) colonies. No erythropoietin was detected in endothelial cell supernatants. This suggests that endothelial cells, a normal component of marrow stroma, play an active role in the modulation of human hematopoietic stem cell growth.

Original languageEnglish (US)
Pages (from-to)553-558
Number of pages6
JournalBlood
Volume63
Issue number3
StatePublished - Jan 1 1984

Fingerprint Dive into the research topics of 'Role of endothelial cells in human hematopoiesis: Modulation of mixed colony growth in vitro'. Together they form a unique fingerprint.

Cite this