Roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of Campylobacter jejuni

Byeonghwa Jeon, Wayne Muraoka, Alexandra Scupham, Qijing Zhang

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Objectives: This study is aimed to determine the role of capsular polysaccharide (CPS) and lipooligosaccharide (LOS) in modulating antimicrobial resistance and natural transformation of Campylobacter jejuni, an important food-borne human pathogen. Methods: A series of C. jejuni mutants, which are defective in either CPS or LOS or both, were constructed. The antimicrobial susceptibility, bacterial surface hydrophobicity, natural transformation frequency and DNA binding and uptake were measured and compared between the mutants and the wild-type strain. Results: Truncation of LOS greatly reduced (8-fold) the intrinsic resistance of C. jejuni to erythromycin, a key antibiotic used for treating human campylobacteriosis, while the loss of CPS did not result in significant changes in the susceptibility to antimicrobial agents. Notably, mutation of LOS also significantly increased (>16-fold) the susceptibility to erythromycin in C. jejuni mutants carrying the A2074G mutation in 23S rRNA. The increased susceptibility to erythromycin in the LOS mutant was probably due to enhanced permeability to this antibiotic, because the LOS mutation rendered the surface of C. jejuni more hydrophobic. Loss of CPS and truncation of LOS increased the transformation frequency by 4- and 25-fold, respectively, and mutation of both CPS and LOS resulted in a 97-fold increase in the transformation frequency. Consistent with the increased transformation frequencies, the CPS and LOS mutants showed enhanced rates of DNA uptake. Conclusions: These results demonstrate that the surface polysaccharides in C. jejuni contribute to the resistance to erythromycin, a clinically important antibiotic, but restrict natural transformation.

Original languageEnglish (US)
Pages (from-to)462-468
Number of pages7
JournalJournal of Antimicrobial Chemotherapy
Volume63
Issue number3
DOIs
StatePublished - 2009
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by the National Institutes of Health grant R01DK063008 and the National Research Initiative competitive grant 2007-35201-18278 from the USDA Cooperative State Research, Education, and Extension Service.

Keywords

  • Competence
  • Drug susceptibility
  • Food safety
  • Macrolides

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