Previous experiments indicated that only a small subset of the ≃24 small nuclear RNAs (snRNAs) in Saccharomyces cerevisiae have binding sites for the Sm antigen, a hallmark of metazoan small nuclear ribonudeoprotcins (snRNPs) involved in premessenger RNA splicing. Antibodies from human serum to Sm proteins were used to show that four snRNAs (snR7, snR14, snR19, and snR20) can be immunopredpitated from yeast extracts. Three of these four, snR7, snR14, and snR20, have been shown to be analogs of mammalian U5, U4, and U2, respectively. Several regions of significant homology to U1 (164 nudeotides) have now been found in cloned and sequenced snR19 (568 nucleotides). These include ten out often matches to the 5′ end of U1, the site known to interact with the 5′ splice site of mammalian introns. Surprisingly, the precise conservation of this sequence precludes perfect complementarity between snR19 and the invariant yeast 5′ junction (GTATGT), which differs from the mammalian consensus at the fourth position (GTPuAGT).