TY - JOUR
T1 - Safety of and Cellular Response to Segmental Bronchoprovocation in Allergic Asthma
AU - Denlinger, Loren C.
AU - Kelly, Elizabeth A.B.
AU - Dodge, Ann M.
AU - McCartney, John G.
AU - Meyer, Keith C.
AU - Cornwell, Richard D.
AU - Jackson, Mary Jo
AU - Evans, Michael D.
AU - Jarjour, Nizar N.
PY - 2013/1/16
Y1 - 2013/1/16
N2 - Rationale: Despite its incorporation into research studies, the safety aspects of segmental allergen bronchoprovocation and differences in cellular response among different allergens have received limited consideration. Methods: We performed 87 segmental challenges in 77 allergic asthma subjects. Allergen dose was based on each subject's response to whole lung allergen challenge. Bronchoalveolar lavage was performed at 0 and 48 hours. Safety indicators included spirometry, oxygen saturation, heart rate, and symptoms. Results: Among subjects challenged with ragweed, cat dander, or house dust mite, there were no differences in safety indicators. Subjects demonstrated a modest oxygen desaturation and tachycardia during the procedure that returned to normal prior to discharge. We observed a modest reduction in forced vital capacity and forced expiratory volume in one second following bronchoscopy. The most common symptoms following the procedure were cough, sore throat and fatigue. Total bronchoalveolar lavage fluid cell numbers increased from 13±4 to 106±108×104 per milliliter and eosinophils increased from 1±2 to 44±20 percent, with no significant differences among the three allergens. Conclusions: In mild allergic asthma, segmental allergen bronchoprovocation, using individualized doses of aeroallergens, was safe and yielded similar cellular responses.
AB - Rationale: Despite its incorporation into research studies, the safety aspects of segmental allergen bronchoprovocation and differences in cellular response among different allergens have received limited consideration. Methods: We performed 87 segmental challenges in 77 allergic asthma subjects. Allergen dose was based on each subject's response to whole lung allergen challenge. Bronchoalveolar lavage was performed at 0 and 48 hours. Safety indicators included spirometry, oxygen saturation, heart rate, and symptoms. Results: Among subjects challenged with ragweed, cat dander, or house dust mite, there were no differences in safety indicators. Subjects demonstrated a modest oxygen desaturation and tachycardia during the procedure that returned to normal prior to discharge. We observed a modest reduction in forced vital capacity and forced expiratory volume in one second following bronchoscopy. The most common symptoms following the procedure were cough, sore throat and fatigue. Total bronchoalveolar lavage fluid cell numbers increased from 13±4 to 106±108×104 per milliliter and eosinophils increased from 1±2 to 44±20 percent, with no significant differences among the three allergens. Conclusions: In mild allergic asthma, segmental allergen bronchoprovocation, using individualized doses of aeroallergens, was safe and yielded similar cellular responses.
UR - http://www.scopus.com/inward/record.url?scp=84872440337&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872440337&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0051963
DO - 10.1371/journal.pone.0051963
M3 - Article
C2 - 23341886
AN - SCOPUS:84872440337
SN - 1932-6203
VL - 8
JO - PloS one
JF - PloS one
IS - 1
M1 - e51963
ER -