Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat

Xunfen Chen; Justin D. Faris; Jinguo Hu; Robert W. Stack; Tika Adhikari; Elias M. Elias; Shahryar F. Kianian; Xiwen Cai

doi:10.1007/s11032-006-9049-7

Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat

Xunfen Chen, Justin D. Faris, Jinguo Hu, Robert W. Stack, Tika Adhikari, Elias M. Elias, Shahryar F. Kianian, Xiwen Cai

Plant Pathology

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Abstract

Fusarium head blight (FHB) is a devastating disease of cultivated wheat worldwide. Partial resistance to FHB has been identified in common wheat (Triticum aestivum L.). However, sources of effective FHB resistance have not been found in durum wheat (T. turgidum L. var. durum). A major FHB resistance quantitative trait loci (QTL), Qfhs.ndsu-3AS, was identified on chromosome 3A of T. dicoccoides, a wild relative of durum wheat. Here, we saturated the genomic region containing the QTL using EST-derived target region amplified polymorphism (TRAP), sequence tagged site (STS), and simple sequence repeat (SSR) markers. A total of 45 new molecular marker loci were detected on chromosome 3A and the resulting linkage map consisted of 55 markers spanning a genetic distance of 277.2 cM. Qfhs.ndsu-3AS was positioned within a chromosomal interval of 11.5 cM and is flanked by the TRAP marker loci, Xfcp401 and Xfcp397.2. The average map distance between the marker loci within this QTL region was reduced from 4.9 cM in the previous study to 3.5 cM in the present study. Comparative mapping indicated that Qfhs.ndsu-3AS is not homoeologous to Qfhs.ndsu-3BS, a major FHB QTL derived from the common wheat cultivar Sumai 3. These results facilitate our efforts toward map-based cloning of Qfhs.ndsu-3AS and utilization of this QTL in durum wheat breeding via marker-assisted selection.

Original language	English (US)
Pages (from-to)	113-124
Number of pages	12
Journal	Molecular Breeding
Volume	19
Issue number	2
DOIs	https://doi.org/10.1007/s11032-006-9049-7
State	Published - Feb 2007

Bibliographical note

Funding Information:
Acknowledgements The authors would like to thank Dr. Shaukat Ali and Jana Hansen for preparing inoculum for FHB evaluation. This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No. 59-0790-3-078. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative.

Keywords

Comparative mapping
Fusarium head blight
QTL
Saturation mapping
Triticum

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{15a076913954430a8d79cb6318627031,

title = "Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat",

abstract = "Fusarium head blight (FHB) is a devastating disease of cultivated wheat worldwide. Partial resistance to FHB has been identified in common wheat (Triticum aestivum L.). However, sources of effective FHB resistance have not been found in durum wheat (T. turgidum L. var. durum). A major FHB resistance quantitative trait loci (QTL), Qfhs.ndsu-3AS, was identified on chromosome 3A of T. dicoccoides, a wild relative of durum wheat. Here, we saturated the genomic region containing the QTL using EST-derived target region amplified polymorphism (TRAP), sequence tagged site (STS), and simple sequence repeat (SSR) markers. A total of 45 new molecular marker loci were detected on chromosome 3A and the resulting linkage map consisted of 55 markers spanning a genetic distance of 277.2 cM. Qfhs.ndsu-3AS was positioned within a chromosomal interval of 11.5 cM and is flanked by the TRAP marker loci, Xfcp401 and Xfcp397.2. The average map distance between the marker loci within this QTL region was reduced from 4.9 cM in the previous study to 3.5 cM in the present study. Comparative mapping indicated that Qfhs.ndsu-3AS is not homoeologous to Qfhs.ndsu-3BS, a major FHB QTL derived from the common wheat cultivar Sumai 3. These results facilitate our efforts toward map-based cloning of Qfhs.ndsu-3AS and utilization of this QTL in durum wheat breeding via marker-assisted selection.",

keywords = "Comparative mapping, Fusarium head blight, QTL, Saturation mapping, Triticum",

author = "Xunfen Chen and Faris, {Justin D.} and Jinguo Hu and Stack, {Robert W.} and Tika Adhikari and Elias, {Elias M.} and Kianian, {Shahryar F.} and Xiwen Cai",

note = "Funding Information: Acknowledgements The authors would like to thank Dr. Shaukat Ali and Jana Hansen for preparing inoculum for FHB evaluation. This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No. 59-0790-3-078. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative.",

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doi = "10.1007/s11032-006-9049-7",

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T1 - Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat

AU - Chen, Xunfen

AU - Faris, Justin D.

AU - Hu, Jinguo

AU - Stack, Robert W.

AU - Adhikari, Tika

AU - Elias, Elias M.

AU - Kianian, Shahryar F.

AU - Cai, Xiwen

N1 - Funding Information: Acknowledgements The authors would like to thank Dr. Shaukat Ali and Jana Hansen for preparing inoculum for FHB evaluation. This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No. 59-0790-3-078. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative.

PY - 2007/2

Y1 - 2007/2

N2 - Fusarium head blight (FHB) is a devastating disease of cultivated wheat worldwide. Partial resistance to FHB has been identified in common wheat (Triticum aestivum L.). However, sources of effective FHB resistance have not been found in durum wheat (T. turgidum L. var. durum). A major FHB resistance quantitative trait loci (QTL), Qfhs.ndsu-3AS, was identified on chromosome 3A of T. dicoccoides, a wild relative of durum wheat. Here, we saturated the genomic region containing the QTL using EST-derived target region amplified polymorphism (TRAP), sequence tagged site (STS), and simple sequence repeat (SSR) markers. A total of 45 new molecular marker loci were detected on chromosome 3A and the resulting linkage map consisted of 55 markers spanning a genetic distance of 277.2 cM. Qfhs.ndsu-3AS was positioned within a chromosomal interval of 11.5 cM and is flanked by the TRAP marker loci, Xfcp401 and Xfcp397.2. The average map distance between the marker loci within this QTL region was reduced from 4.9 cM in the previous study to 3.5 cM in the present study. Comparative mapping indicated that Qfhs.ndsu-3AS is not homoeologous to Qfhs.ndsu-3BS, a major FHB QTL derived from the common wheat cultivar Sumai 3. These results facilitate our efforts toward map-based cloning of Qfhs.ndsu-3AS and utilization of this QTL in durum wheat breeding via marker-assisted selection.

AB - Fusarium head blight (FHB) is a devastating disease of cultivated wheat worldwide. Partial resistance to FHB has been identified in common wheat (Triticum aestivum L.). However, sources of effective FHB resistance have not been found in durum wheat (T. turgidum L. var. durum). A major FHB resistance quantitative trait loci (QTL), Qfhs.ndsu-3AS, was identified on chromosome 3A of T. dicoccoides, a wild relative of durum wheat. Here, we saturated the genomic region containing the QTL using EST-derived target region amplified polymorphism (TRAP), sequence tagged site (STS), and simple sequence repeat (SSR) markers. A total of 45 new molecular marker loci were detected on chromosome 3A and the resulting linkage map consisted of 55 markers spanning a genetic distance of 277.2 cM. Qfhs.ndsu-3AS was positioned within a chromosomal interval of 11.5 cM and is flanked by the TRAP marker loci, Xfcp401 and Xfcp397.2. The average map distance between the marker loci within this QTL region was reduced from 4.9 cM in the previous study to 3.5 cM in the present study. Comparative mapping indicated that Qfhs.ndsu-3AS is not homoeologous to Qfhs.ndsu-3BS, a major FHB QTL derived from the common wheat cultivar Sumai 3. These results facilitate our efforts toward map-based cloning of Qfhs.ndsu-3AS and utilization of this QTL in durum wheat breeding via marker-assisted selection.

KW - Comparative mapping

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KW - QTL

KW - Saturation mapping

KW - Triticum

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ER -

Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat

Abstract

Bibliographical note

Keywords

UN SDGs

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