Scaling behavior in mitochondrial redox fluctuations

V. Krishnan Ramanujan, Gabriel Biener, Brian A. Herman

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Scale-invariant long-range correlations have been reported in fluctuations of time-series signals originating from diverse processes such as heart beat dynamics, earthquakes, and stock market data. The common denominator of these apparently different processes is a highly nonlinear dynamics with competing forces and distinct feedback species. We report for the first time an experimental evidence for scaling behavior in NAD(P)H signal fluctuations in isolated mitochondria and intact cells isolated from the liver of a young (5-month-old) mouse. Time-series data were collected by two-photon imaging of mitochondrial NAD(P)H fluorescence and signal fluctuations were quantitatively analyzed for statistical correlations by detrended fluctuation analysis and spectral power analysis. Redox [NAD(P)H/NAD(P)+] fluctuations in isolated mitochondria and intact liver cells were found to display nonrandom, long-range correlations. These correlations are interpreted as arising due to the regulatory dynamics operative in Krebs' cycle enzyme network and electron transport chain in the mitochondria. This finding may provide a novel basis for understanding similar regulatory networks that govern the nonequilibrium properties of living cells.

Original languageEnglish (US)
Pages (from-to)L70-L72
JournalBiophysical journal
Volume90
Issue number10
DOIs
StatePublished - May 2006

Bibliographical note

Funding Information:
This work was partially supported by the grant 2RO1–AG007218-18A1 from National Institutes of Health/National Institute on Aging to B.H.

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