Scanning tunnelling microscopy of tobacco mosaic virus on evaporated and sputter‐coated palladium/gold substrates

J. G. Mantovani, D. P. Allison, R. J. Warmack, T. L. Ferrell, J. R. Ford, R. E. Manos, J. R. Thompson, B. B. Reddick, K. Bruce Jacobson

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27 Scopus citations

Abstract

We present scanning tunnelling microscope (STM) images of untreated tobacco mosaic virus (TMV) deposited on thermally evaporated and on sputter‐coated palladium/gold 40:60 (Pd/Au) substrates, and imaged under ordinary atmospheric conditions. The TMV imaged on both evaporated and sputter‐coated substrates was consistently several times wider than the known diameter of the virus. TMV on evaporated Pd/Au became overcoated with Pd/Au material during sample preparation and appeared elevated in STM images, whereas TMV on sputter‐coated Pd/Au appeared as depressions. When naked TMV were intentionally overcoated with Pd/Au, the STM images were found to be similar to those for TMV on evaporated Pd/Au. 1990 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)109-116
Number of pages8
JournalJournal of Microscopy
Volume158
Issue number1
DOIs
StatePublished - Apr 1990
Externally publishedYes

Keywords

  • Scanning tunnelling microscope
  • evaporated substrate
  • palladium/gold
  • sputter‐coated substrate
  • tobacco mosaic virus

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