Scutellarin suppresses patient-derived xenograft tumor growth by directly targeting AKT in esophageal squamous cell carcinoma

Feifei Liu, Xueyin Zu, Xiaomeng Xie, Yuanyuan Zhang, Kangdong Liu, Hanyong Chen, Ing Wang, Ann M. Bode, Zigang Dong, Dong Joon Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Scutellarin is a flavonoid compound that is found in Scutellaria barbata. It has been reported to exhibit anticancer and anti-inflammation activities. However, the anticancer properties of scutellarin and its molecular targets have not been investigated in esophageal squamous cell carcinoma (ESCC). In the current study, we report that scutellarin is a potential AKT inhibitor that suppresses patient-derived xenograft ESCC tumor growth. To identify possible molecular targets of scutellarin, potential candidate proteins were screened by an in vitro kinase assay and Western blotting. We found that scutellarin directly binds to the AKT1/2 proteins and inhibits activities of AKT1/2 in vitro. The AKT protein is activated in ESCC tissues and knockdown of AKT significantly suppresses growth of ESCC cells. Scutellarin significantly inhibits anchorage-dependent and independent cell growth and inducesG2 phase cell-cycle arrest in ESCC cells. The inhibition of cell growth by scutellarin is dependent on the expression of the AKT protein. Notably, scutellarin strongly suppresses patient-derived xenograft ESCC tumor growth in an in vivo mouse model. Taken together, our data suggest that scutellarin is a novel AKT inhibitor thatmayprevent progression of ESCC.

Original languageEnglish (US)
Pages (from-to)849-860
Number of pages12
JournalCancer Prevention Research
Volume12
Issue number12
DOIs
StatePublished - 2019

Bibliographical note

Funding Information:
This work was supported by Henan Joint Fund, National Natural Science Foundation China (NSFC; grant number U1804196 and 81572812), and Key program of Henan Province, China (grant number 161100510300).

Publisher Copyright:
© American Association for Cancer Research.

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