Selection of Candida albicans trisomy during oropharyngeal infection results in a commensal-like phenotype

Anja Forche, Norma V. Solis, Marc Swidergall, Robert Thomas, Alison Guyer, Annette Beach, Gareth A. Cromie, Giang T. Le, Emily Lowell, Norman Pavelka, Judith Berman, Aimeé M. Dudley, Anna Selmecki, Scott G. Filler

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

When the fungus Candida albicans proliferates in the oropharyngeal cavity during experimental oropharyngeal candidiasis (OPC), it undergoes large-scale genome changes at a much higher frequency than when it grows in vitro. Previously, we identified a specific whole chromosome amplification, trisomy of Chr6 (Chr6x3), that was highly overrepresented among strains recovered from the tongues of mice with OPC. To determine the functional significance of this trisomy, we assessed the virulence of two Chr6 trisomic strains and a Chr5 trisomic strain in the mouse model of OPC. We also analyzed the expression of virulence-associated traits in vitro. All three trisomic strains exhibited characteristics of a commensal during OPC in mice. They achieved the same oral fungal burden as the diploid progenitor strain but caused significantly less weight loss and elicited a significantly lower inflammatory host response. In vitro, all three trisomic strains had reduced capacity to adhere to and invade oral epithelial cells and increased susceptibility to neutrophil killing. Whole genome sequencing of pre- and post-infection isolates found that the trisomies were usually maintained. Most post-infection isolates also contained de novo point mutations, but these were not conserved. While in vitro growth assays did not reveal phenotypes specific to de novo point mutations, they did reveal novel phenotypes specific to each lineage. These data reveal that during OPC, clones that are trisomic for Chr5 or Chr6 are selected and they facilitate a commensal-like phenotype.

Original languageEnglish (US)
Article numbere1008137
JournalPLoS genetics
Volume15
Issue number5
DOIs
StatePublished - May 2019
Externally publishedYes

Bibliographical note

Funding Information:
AMD, GC, EL, and AF were funded by National Institutes of Health grant R15 AI090633 to AF. This work was supported in part by National Institutes of Health grants R01DE022600 and R01AI124566 to SGF., and grant K99DE026856 to MS. AMS was funded by Nebraska Tobacco Settlement Biomedical Research Development New Initiative Grant (LB692), NE-EPSCoR First Award, Nebraska Department of Health and Human Services (LB506-2017-55), CURAS Faculty Research Fund Award, and an NIH-NCRR COBRE grant P20RR018788 sub-award. JB was funded by an European Research Council Advanced Award 340087 (RAPLODAPT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2019, Public Library of Science. All rights reserved.

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