Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation

Mohammad Rashidian, Jonathan K. Dozier, Stepan Lenevich, Mark D. Distefano

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protein modification provides an alternative to the commonly employed Cu(i)-catalyzed click reaction.

Original languageEnglish (US)
Pages (from-to)8998-9000
Number of pages3
JournalChemical Communications
Volume46
Issue number47
DOIs
StatePublished - Dec 21 2010

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